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基于酸功能化的Eu (III) 基金属有机框架的超敏感检测Vibrio parahaemolyticus

Jingyi Xiao¹, Yi Ren¹, Menglong Liu¹

¹College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province. Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities. Jinzhou, Liaoning, 121013, China.

Analytica chimica acta

|February 21, 2025

概括

这项研究开发了一种新型的比度光探针,用于检测海鲜中的Vibrio parahaemolyticus. 具有阿普塔默功能的传感器为食品安全提供快速,灵敏和现场的病原体检测.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
食品科学食品科学食品科学

背景情况:

维亚血解菌 (Vibrio parahaemolyticus) 是水生产品中一种主要的食源性病原体.
准确和快速的检测方法对于食品安全至关重要.
现有的检测方法可能耗时,需要专门的设备.

研究的目的:

开发一种比度光探针,用于敏感检测Vibrio parahaemolyticus.
创建一个便携式传感器,以便在现场快速检测病原体.
加强食品安全监测系统.

主要方法:

采用了APTAMER功能化的磁纳米粒子,用于特定捕获V. parahaemolyticus.
采用欧洲 (III) 基金属有机框架 (Eu-MOF) 与酸通过糖脂相互作用进行双重识别.
量化V. parahaemolyticus使用比例光强度测量.
开发了一种基于智能手机的便携式传感器,用于现场分析.

主要成果:

传感器实现了从77到7.7×10^7 CFU/mL的广泛检测范围.
证明了1CFU/mL的低检测极限.
该方法在真实食品样本中显示出成功的应用.
便携式传感器证实了快速有效的病原体检测能力.

结论:

开发的比度光探针为V. parahaemolyticus检测提供了一种敏感和特定的方法.
便携式智能手机传感器可以在现场快速识别病原体.
这项技术对食品安全和环境监测做出了重大贡献.

关键词:

亚普特美尔 (Aptamer) 是一种药物.酸功能化的酸功能化欧洲农业基金 (Eu-MOF) 是一个基金.相对测量光度的光度.这种病毒是Vibrio parahaemolyticus.

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