相关实验视频
Updated: May 26, 2025

08:01
The Soft Agar Colony Formation Assay
Published on: October 27, 2014
111.3K
长非编码RNAPRKG1-AS1在肺腺癌中促进细胞增殖和迁移
Tong Jiao1, Haimei Wen1, Lizhong Zeng1
1Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, No. 157, Xiwu Road, Xincheng District, Xi'an, Shaanxi, 710000, P.R. China.
Respiratory research
|February 21, 2025
概括
在肺腺癌 (LUAD) 中,cGMP依赖的蛋白质激酶I反感RNA 1 (PRKG1-AS1) 过度表达,与预后不佳相关. 这种长长的非编码RNA可能充当瘤基因,影响LUAD的发展和患者的治疗结果.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 肺腺癌 (LUAD) 是癌症死亡的主要原因.
- 确定新的预后生物标志物和LUAD的治疗点至关重要.
- 在LUAD中PRKG1-AS1等长非编码RNA (lncRNAs) 的作用是一个正在积极研究的领域.
研究的目的:
- 调查cGMP依赖蛋白激酶I抗理性RNA1 (PRKG1-AS1) 在肺腺癌 (LUAD) 中的作用和预后意义.
- 探索PRKG1-AS1表达和LUAD患者的临床结果之间的相关性.
- 阐明在LUAD中PRKG1-AS1功能的潜在分子机制.
主要方法:
- 对癌症基因组图谱 (TCGA) 数据库和临床LUAD组织样本的分析.
- 定量实时PCR用于评估PRKG1-AS1表达水平.
- 在PRKG1-AS1倒置后进行细胞增殖和转移测定.
- 基因组丰富分析 (GSEA) 和与蛋白质编码基因的相关性分析.
主要成果:
- 发现,与正常组织相比,PRKG1-AS1在LUAD组织中显著过度表达.
- 高PRKG1-AS1表达与预后不佳密切相关,并作为LUAD患者的独立预后因素.
- 抑制PRKG1-AS1抑制了LUAD细胞的增殖和转移.
- PRKG1-AS1与dickkopf-1 (DKK1) 呈正相关性,这是另一种与LUAD预后不佳相关的瘤基因.
结论:
- 在LUAD中,PRKG1-AS1充当瘤基因,促进瘤的进展.
- PRKG1-AS1对于LUAD患者来说是一个潜在的有价值的预后生物标志物.
- 向PRKG1-AS1可能是LUAD的一种新疗法策略.
更多相关视频
相关概念视频
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
The Ras Gene
6.1K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
6.1K
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
The Retinoblastoma Gene
4.0K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.0K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K

