相关实验视频

Updated: May 26, 2025

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Published on: April 26, 2019

由Huc-MSCs衍生的外体通过通过miR-24-3p/STING轴调节巨细胞极化来缓解非酒精性脂肪肝炎

Wei Jiang^1,2, Qingmin Zeng^1,2, Chang-Hai Liu^1,2

¹Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, 610041, China.

Stem cell research & therapy

|February 21, 2025

概括

人类带介质干细胞衍生的外体细胞 (huc-MSCs外体细胞) 通过调节巨细胞极化来治疗非酒精性脂肪肝炎 (NASH) 是有前途的. 这项研究强调了miR-24-3p作为这些外体对NASH治疗效果的关键因素.

科学领域:

肝病学和再生医学肝病学和再生医学
细胞生物学和免疫学

背景情况:

由于缺乏有效的治疗方法,非酒精性脂肪肝炎 (NASH) 存在重大未满足的医疗需求.
人类带介质干细胞衍生的外体细胞 (huc-MSCs外体细胞) 在调节新陈代谢和免疫反应方面表现出潜在的潜力,为NASH治疗进行调查是有必要的.

研究的目的:

在NASH的小鼠模型中研究huc-MSCs外体的治疗疗效.
阐明 huc-MSCs 外基因组对NASH进展产生影响的潜在机制,重点关注免疫细胞调节.

主要方法:

纳什在C57BL/6J小鼠中被诱导使用西方饮食12周.
在饮食期间,小鼠接受了huc-MSCs外体组 (MSCs-exo),评估包括体重,血糖,胰岛素水平和肝脏病理.
机制研究涉及流细胞计和免疫光染色,以分析巨细胞两极分化和基因表达.

主要成果:

使用MSCs-exo显著降低了肝脏脂肪,炎症和纤维化,改善了NASH小鼠的代谢参数.
外体细胞减少了肝脏巨细胞的积累,促进了从M1向M2极化转变.
在MSCs-exo中确定了miR-24-3p的高表达,可能准STING基因以调节巨细胞两极分化并增强治疗效益.

结论:

MSCs-exo对NASH的治疗作用可能是由miR-24-3p诱导的M2巨细胞两极化通过STING向介导的.
这些发现为MSCs-exo在治疗NASH的临床应用提供了强有力的科学依据.

关键词:

外基因组是外基因组的组成部分.巨细胞是一个巨细胞.介质细胞干细胞介质细胞干细胞非酒精性脂肪肝炎是什么?刺痛是一种刺痛.在 miR-24-3p中使用.

更多相关视频

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Author Spotlight: Establishing MASLD Cell Models for Investigating Disease Mechanisms and the Lipid-Lowering Effects of Koumiss

Published on: July 19, 2024

Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice

Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice

Published on: December 2, 2022

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...