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层层叠叠的膜的最外层阴离子表面电荷阻止了内皮细胞迁移,从而在三维组织中进行细胞细分

Jinfeng Zeng^1,2, Sven Heilig³, Matthias Ryma³

¹College of Textiles, Donghua University, Shanghai, 201620, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|February 22, 2025

概括

这项研究使用聚-l-lysine 和 dextran 开发了人工底层膜 (A-BM). 这些A-BM有效地控制细胞迁移,使有组织的3D组织工程能够用于药物测试.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
细胞生物学细胞生物学

背景情况:

生物组织的功能依赖于有组织的细胞结构.
传统的3D细胞封装方法与细胞迁移作斗争,阻碍了组织的复杂性.
底层膜 (BMs) 对于组织中的机械支和细胞组织至关重要.

研究的目的:

设计模仿自然BM屏障功能的人工地下膜 (A-BMs).
研究表面电荷在调节内皮细胞 (EC) 迁移中的作用.
为组织工程开发一种创建有组织的3D血管通道的方法.

主要方法:

制造多层纳米薄膜使用层层组装的聚-l-氨酸 (PLL) 和氨酸 (Dex).
利用键进行纳米薄膜组装,创建带正电荷的最外围表面.
在14天内评估A-BM屏障特性和EC迁移抑制.
工程3D组织血管通道使用可适应形状的PLL/Dex纳米膜.

主要成果:

与其他A-BM相比,制造的PLL/Dex A-BM表现出增强的屏障特性.
带正电荷的外围表面有效地抑制了内皮细胞的发芽和迁移,持续了14天.
通过使用可适应形状的纳米膜成功设计了3D有组织的血管通道.
A-BMs支持细胞组织,模仿自然的BM形成时间线.

结论:

带正电荷的PLL/Dex纳米膜可以有效调节细胞迁移,作为功能性的A-BMs.
这种方法为设计有组织的3D组织模型提供了一个新的策略.
工程平台适用于药物和药物输送载体的体外透性测定.

关键词:

欧洲共同体的芽芽.底层膜的底层膜.细胞迁移细胞迁移有图案的血管组织.有正电荷的纳米片.

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