CREB1控制了人类支气管细胞和肺损伤的1 - 尼托烯介导的亡中的线粒体功能障碍
Min Jeong Kim1, Hee Jung Choi1, Ha Yeong Kang1
1School of Food Science and Biotechnology, Kyungpook National University, Republic of Korea.
The Science of the total environment
|February 22, 2025
概括
亚多环芳 (Nitro-PAHs),就像柴油排气中的1-NP一样,会导致肺部损伤. 这项研究表明,1-NP通过线粒体功能障碍和CREB1酸化诱导细胞死亡,为呼吸系统疾病治疗提供了标.
科学领域:
- 环境毒理学环境毒理学
- 分子生物学分子生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 空气中携带的多环芳 (nitro-PAHs),特别是来自柴油废气颗粒 (DEP) 的1-NP,与呼吸系统疾病有关.
- 关于1-NP呼吸毒性的分子机制的理解有限,包括线粒体功能障碍和肺细胞死亡中的基因表达.
研究的目的:
- 为了研究1-NP暴露,肺细胞死亡,线粒体功能障碍和基因表达之间的关系.
- 阐明CREB1酸化在人类支气管上皮细胞和小鼠模型中的1-NP诱导毒性的作用.
主要方法:
- 在体外对人类支气管上皮细胞进行测试,以评估线粒体功能和亡.
- 蛋白质组分析以确定关键信号通路.
- 在体内研究中,使用了通过鼻内灌注暴露于1-NP的小鼠模型.
主要成果:
- 1-NP暴露增加了亡标记物 (caspase-3,PARP),破坏了线粒体晶状体,并增加了ROS和VDAC1的表达.
- 发现CREB1酸化对1-NP毒性有保护作用;CREB1倒置恶化了线粒体功能障碍和细胞死亡.
- 在体内,1-NP诱导肺炎 (CCL2,IL-1β,CXCL1),纤维化,粘素过度表达和DNA损伤 (8-OHdG).
结论:
- 1-NP暴露会触发CREB1酸化,线粒体功能障碍,亡以及包括炎症和纤维化在内的肺病理.
- 这些发现强调CREB1酸化是减轻1-NP毒性的关键因素,并建议在呼吸系统疾病中潜在的治疗点.
关键词:
一-尼特罗皮尔烯 (Nitropyrene) 是一种1 - 尼特罗皮烯.空气污染 大气污染细胞灭亡 (apoptosis) 是一种死亡的过程.在CREB1中,CREB1是指CREB1,CREB1是指CREB1,CREB1是指CREB1.肺部崩导致肺部崩.线粒体功能障碍 线粒体功能障碍更多相关视频
10:37Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice
Published on: January 16, 2015
13.2K
19:44Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
Published on: May 30, 2012
18.7K
相关概念视频
01:31Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
02:26NF-κB-dependent Signaling Pathway
7.2K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.2K
01:46Electron Transport Chain: Complex I and II
10.2K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
10.2K
01:45Mitochondrial Membranes
7.2K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
7.2K