2型糖尿病的低血糖控制会影响白细胞的氧气消耗率,OXPHOS复合物的含量和中性粒细胞与内皮细胞的相互作用
Julia Cacace1, Clara Luna-Marco2, Alberto Hermo-Argibay1
1Service of Endocrinology and Nutrition, Foundation for the Promotion of Health and Biomedical Research in the Valencian Region (FISABIO), University Hospital Doctor Peset, Valencia, Spain.
Redox biology
|February 22, 2025
概括
2型糖尿病 (T2D) 中血糖控制不良会损害线粒体功能,并增加外周血液单核细胞 (PBMC) 中的氧化应激. 这种功能障碍与T2D患者的炎症和血管疾病风险更高有关.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 代谢疾病 代谢疾病
背景情况:
- 线粒体功能障碍和活性氧物种 (ROS) 生产增加是2型糖尿病 (T2D) 的标志.
- 周围血液单核细胞 (PBMC) 在T2D相关的炎症中起作用.
- 血糖控制不良会加剧T2D并发症和并发症.
研究的目的:
- 研究血糖控制对PBMC氧气消耗率 (OCR),氧化酸化 (OXPHOS) 复合体和T2D炎症的影响.
- 为了将血糖控制 (HbA1c水平) 与线粒体功能和炎症标志物相关联.
主要方法:
- 招募了181名受试者:79名健康对照组,64名T2D患者具有良好的血糖控制 (HbA1c<7%),38名T2D患者具有不良的血糖控制 (HbA1c>7%).
- 测量了PBMC基底和最大OCR,备用呼吸能力,线粒体ROS水平和OXPHOS复合物的表达.
- 评估了中性粒细胞与内皮细胞的相互作用以及髓氧化酶 (MPO),ICAM-1和VCAM-1的水平.
主要成果:
- 血糖控制较差的患者 (HbA1c>7%) 与对照组和控制良好的T2D患者相比,表现出较低的基础和最大OCR,以及较低的备用呼吸能力.
- 线粒体ROS水平在T2D患者中显著升高,特别是在控制不良的组中.
- 低血糖控制与线粒体复合体III和V功能下降,中性粒细胞内皮相互作用增加,MPO,ICAM-1和VCAM-1水平相关.
结论:
- 在T2D中血糖控制不良会影响PBMC线粒体呼吸和OXPHOS复合体完整性.
- 这些线粒体变化与炎症增加和内皮激活相关.
- 由于这些细胞和分子变化,血糖控制不良的T2D患者面临血管疾病发展的风险增加.
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