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来自Bacillus thuringiensis的杀虫剂蛋白质对白有毒,Bemisia tabaci有毒

Clebson S Tavares¹, Xinyue Wang¹, Saptarshi Ghosh¹

¹Department of Entomology and Nematology, University of Florida, PO Box 110620, Gainesville, FL 32611, USA.

Journal of invertebrate pathology

|February 22, 2025

概括

新的Bacillus thuringiensis (Bt) 杀虫剂蛋白对主要农业害虫白 (Bemisia tabaci) 显示有毒性. 这些发现为新型生物杀虫剂管理白种群和减少作物中的病毒传播提供了潜力.

科学领域:

农业昆虫学农业昆虫学
分子昆虫学分子昆虫学
生物技术是生物技术.

背景情况:

白 (Bemisia tabaci) 由于养损害和病毒传播,对全球农业构成重大威胁.
在B. tabaci中广泛存在的抗昆虫剂耐药性,需要采用替代的害虫防治策略.
来自Bacillus thuringiensis (Bt) 的杀虫剂蛋白质是新型生物杀虫剂的潜在来源,但很少有人对B. tabaci.有活力.

研究的目的:

选一个由11个Bt衍生的杀虫剂蛋白质组成的小组,以检测中东-小亚洲1 (MEAM1) B. tabaci. 的神秘物种B. tabaci. 的毒性.
确定有效管理B. tabaci种群的新型Bt蛋白质.
为了研究B. tabaci活性蛋白对昆虫肠道的作用模式.

主要方法:

通过养生物试验,对11种纯化的Bt衍生农药蛋白进行了针对成年B. tabaci (MEAM1) 的测试.
对于具有显著死亡率的蛋白质,确定了致命度 (LC50) 值.
使用传输电子显微镜检查Tpp78Aa1蛋白对白肠表皮的影响.

主要成果:

三种杀虫剂蛋白,Tpp78Aa1,Tpp78Ba1和Cry1Ca,对B. tabaci表现出显著的毒性.
确定LC50值为Tpp78Aa1的99微克/毫升,Tpp78Ba1的96微克/毫升,Cry1Ca.的351微克/毫升.
Tpp78Aa1诱导了白肠中的刷边缘和微的破坏,与Bt蛋白的作用模式一致.

结论:

Tpp78Aa1,Tpp78Ba1和Cry1Ca代表了Bemisia tabaci综合管理的有希望的新工具.
这些Bt衍生蛋白可能有助于减少作物损失和白传播植物病毒的发生率.
对这些蛋白质的进一步研究可能会导致为可持续农业开发有效的生物杀虫剂.

关键词:

细菌杀虫剂蛋白质是一种细菌杀虫剂蛋白质.贝米西亚烟草 (Bemisia tabaci) 是一种烟草.在Cry1CaCa.在MEAM1中.Tpp7878 在线播放

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