在小鼠中,BHPF通过扰乱母细胞转化和线粒体功能来抑制早期胚胎发育
Zhiming Ding1, Hongzhen Ruan1, Yujie Wang1
1Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, No.218 Jixi Road, Hefei, 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, No.81 Meishan Road, Hefei, 230032, China.
概括
-9-双 (BHPF) 通过破坏关键遗传过程,线粒体功能,并导致DNA损伤,破坏小鼠胚胎早期发育. 这引发了人们对其作为双A (BPA) 替代品的安全性的担忧.
科学领域:
- 生殖毒理学 生殖毒理学
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
背景情况:
- -9-双 (BHPF) 是消费品中常见的双A (BPA) 替代品.
- BHPF是一种已知的内分泌干扰物,但其对早期胚胎发育的影响尚不清楚.
研究的目的:
- 在实验室中研究BHPF暴露对小鼠早期胚胎发育的影响.
- 阐明BHPF诱导的发育毒性背后的分子机制.
主要方法:
- 在实验室培养的小鼠胚胎,接受了不同度的BHPF治疗.
- 使用免疫光,细胞活着染色和转录组测序.
- 分析胚胎基因组激活,母体转变为胚胎,线粒体功能,氧化应激,DNA损伤和基因组修饰.
主要成果:
- 暴露于BHPF导致了胚胎早期显著的发育停止.
- 转录组分析显示,胚胎细胞基因组活化受损和母体mRNA降解,破坏了母体到胚胎细胞的过渡.
- 在接受BHPF治疗的胚胎中观察到线粒体功能障碍,氧化应激增加,DNA损伤和改变的基因组修饰.
结论:
- BHPF对早期胚胎发育构成风险.
- 研究结果表明,BHPF破坏了关键的发育过程,包括遗传激活,线粒体功能和表观遗传调节.
- 对BHPF作为BPA替代品的安全性存在担忧是有道理的.
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