一个试验式空运反应堆集成了一个低剪切旋转微气泡发生器用于使用草作为基板的酵母生产
Yifan Bu1, Hang Xiao2, Haidong Zhang2
1Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao New Energy Shandong Laboratory, Shandong Energy Institute, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China; University of Chinese Academy of Sciences, Beijing 100080, China.
Bioresource technology
|February 22, 2025
概括
一个带有微气泡的新型空运循环反应堆 (ALR) 通过改善质量转移和混合来增强草的酵母生产. 与传统方法相比,这种生物反应器的设计可提高可行的细胞产量超过40%.
科学领域:
- 生物化学工程 生物化学工程
- 生物处理技术 生物处理技术
- 发酵科学 发酵科学
背景情况:
- 传统的空运循环反应堆 (ALR) 在质量转移和混合效率方面面临限制,特别是在使用像草这样的固体基板时.
- 改善这些参数对于提高微生物生产率和优化发酵过程至关重要.
研究的目的:
- 设计和验证一个试点规模的空运循环反应堆 (ALR) 与一个低剪切旋转微气泡发生器集成.
- 用草作为基质来增强质量转移,混合和酵母 (Candida utilis) 生产.
- 调查新生物反应器对固体基质特性和细胞活性的影响.
主要方法:
- 开发和实施试点规模的ALR与低剪切旋转微气泡发生器相结合.
- 在增强外部循环的空气--液体系统中探索水力动力学.
- 微泡大小 (小于150微米) 的研究及其对质量转移和固体基板性能的影响 (子d50).
- 种植Candida utilis (C. utilis) 来评估酵母生产和细胞活力.
主要成果:
- 由于血液循环和微气泡的增强,质量转移显著增加了11.5倍.
- 长期操作后,粒大小 (d50) 减少了大约3微米,这表明基板发生了修改.
- 与传统的ALR相比,Candida utilis培养产生了40.8%的可活细胞,具有持续的细胞活性.
- 综合生物反应器表现出优异的混合,质量转移,阻塞性和低剪切条件.
结论:
- 新的微泡增强ALR设计有效提高了酵母的生产力,并为固体基板利用提供了可行的解决方案.
- 双循环生物反应器在混合,质量转移和低剪切方面的能力非常有利于生物发酵.
- 这项研究验证了将微泡生成集成到ALR中用于先进生物处理应用的有效性.
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