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可靠的方法来确定PBAT在无氧条件下的生物转化

Alba Trueba-Santiso¹, Reinhard Wimmer², Mathias Eskildsen²

¹CRETUS, Department of Chemical Engineering, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, Galicia, Spain.

Bioresource technology

|February 22, 2025

概括

可生物降解的塑料,如聚乙烯基合甲酸 (PBAT),在热性无氧消化 (AD) 中显示部分分解. 像酸和甲酸这样的单体积聚,表明尽管有稳定的微生物群落,但降解不完整.

科学领域:

环境微生物学环境微生物学
聚合物科学聚合物科学
生物技术是生物技术.

背景情况:

可生物降解塑料可以改善无氧消化 (AD) 中的食品废物管理.
在热友AD条件下 (55°C) 塑料如聚乙烯基合甲基甲基甲基甲基甲基甲基 (PBAT) 的行为尚不清楚.
之前的研究报告说,由于分析方法的多样性,塑料生物转化结果不一致.

研究的目的:

为了研究PBAT在热友性无氧消化中的生物转化.
量化降解产品并评估对微生物群落的影响.
建立一个可靠的方法来评估塑料的生物转化.

主要方法:

在55°C的温度下对PBAT进行热友性无氧消化 (AD).
使用1HNMR光谱学量化PBAT单体 (1,4-butanediol,酸 (AA),铁酸 (TPA)) 的量化.
微生物社区稳定性的分析.

主要成果:

1,4-二醇被迅速降解.
亚酸 (AA) 和铁酸 (TPA) 单体,随着时间的推移而积累.
估计PBAT生物转化率为11.1 ± 1.9% (TPA) 和10.1 ± 2.3% (AA).
核心微生物群落在整个过程中保持稳定.

结论:

在热友性AD中,PBAT经历了部分生物转化,观察到单体积累.
微生物群体具有内在的水解能力,尽管积聚了单体,但它们仍然保持稳定.
开发的1H NMR工作流提供了一种可靠的方法来评估AD系统中的塑料生物转化.

关键词:

(1) H NMR 的时间.无氧消化消化无氧消化转基因组学是指转基因组学.在PBAT和PBAT之间塑料塑料的塑料的热友性热友性热友性热友性

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