相关实验视频

Updated: May 26, 2025

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

尼日利亚三L.种子提取物减轻氧化应激诱导的细胞衰老和色细胞的功能障碍

Ben Niu¹, Xiaohong An², Yongmei Chen³

¹School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Wuxi People's Hospital, Nanjing Medical University, Wuxi 214023, China.

Chinese journal of natural medicines

|February 22, 2025

概括

尼吉拉三 (Nigella sativa) 提取物 (HZC) 增强黑色素的产生,并保护黑色素细胞免受氧化应激和衰老. 这种传统疗法通过增强皮肤细胞功能和防止过早衰老,对白风治疗有希望.

科学领域:

皮肤病学皮肤病学
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

尼日利亚白种子传统上在中国医学中用于治疗白风.
黑色素细胞功能障碍和氧化应激有助于白风的发病.

研究的目的:

调查乙醇性尼日利亚三叶草L.提取物 (HZC) 对黑色素细胞中黑色素生成和氧化应激的影响.
评估HZC在缓解细胞衰老和功能障碍方面的潜力.

主要方法:

提取和冷凍 Nigella sativa L. 的乙醇分數.
在体外测定包括MTT,铁酶活性,黑色素含量,划痕测定,SA-β-染,ELISA和西部涂抹.
对信号通路 (MAPK,PKA,Wnt,Nrf2-ARE) 的分析.

主要成果:

HZC显著增加了B16F10细胞中的铁酶活性和黑色素含量.
HZC提高了关键的黑色素蛋白的表达 (Tyr, Mitf, gp100).
通过激活Nrf2-ARE通路,HZC减轻了H2O2诱导的氧化应激,减少了细胞衰老,并改善了黑色素细胞功能.

结论:

尼日利亚三L.提取物 (HZC) 通过MAPK,PKA和Wnt途径促进黑色素的产生.
HZC通过Nrf2-ARE通路保护黑色素细胞免受氧化应激和衰老.
HZC通过增强黑色素细胞功能和弹性来证明白风的治疗潜力.

关键词:

细胞衰老细胞衰老黑色素是黑色素的一种.尼吉拉·萨蒂瓦L.种子提取物 (HZC)这就是ROSOS ROS.脊髓灰质炎 (vitiligo) 是一种疾病.

更多相关视频

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Published on: July 8, 2020

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

相关概念视频

Mitochondria

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...

Nucleotide Excision Repair

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...