多种结构上不同的战争的共同分子形状在引起皮肤化学水伤害方面具有共同的分子形状
Ritesh Kumar Srivastava1, Suhail Muzaffar1, Jasim Khan1
1UAB Research Center of Excellence in Arsenicals, Department of Dermatology, University of Alabama at Birmingham, Volker Hall - 509, 1670 University Blvd., Birmingham, AL, 35294-0019, USA.
Scientific reports
|February 22, 2025
概括
接触DPCA和DPCYA等某些素会导致严重的皮肤损伤,炎症和细胞死亡. 这些战斗剂激活炎症和展开的蛋白质反应,破坏皮肤屏障的完整性.
科学领域:
- 毒理学 毒理学 毒理学
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
背景情况:
- 众所周知,包括lewisite在内的素会导致严重的皮肤损伤.
- 了解结构上不同的素造成的皮肤损伤的分子机制对于开发对策至关重要.
研究的目的:
- 描述由二甲素 (DPCA),二甲素 (DPCYA) 和二甲素 (DECA) 诱导的皮肤损伤的分子致病性.
- 为了比较这些素在小鼠皮肤上的毒性作用和细胞反应.
主要方法:
- 通过皮肤暴露小鼠皮肤DPCA,DPCYA和DECA.
- 临床评分 (德赖兹评分,皮肤厚度) 和皮肤组织组织组织学分析.
- 评估免疫细胞透 (巨细胞,中性粒细胞) 和微小囊的形成.
- 对炎症反应,活性氧物种 (ROS) 和未折叠蛋白质反应 (UPR) 信号的RT-PCR分析.
- 对紧张和粘附结蛋白 (Yap/ZO-1,Yap/α-Catenin) 的评估.
主要成果:
- 与DECA不同的是,DPCA和DPCYA诱导了显著的红血,水和痕形成.
- 组织学揭示了炎症,免疫细胞透 (巨细胞然后是中性粒细胞) 和表皮亡.
- 在暴露于DPCA/DPCYA的皮肤中观察到ROS和UPR信号的增加.
- DECA暴露也增加了ROS和改变了UPR.
- 紧张和粘附结的破坏导致了角质细胞的亡.
结论:
- 通过炎症和细胞应激通路,DPCA和DPCYA会引起显著的皮肤损伤.
- 这些质物质通过影响结 protein 破坏皮肤屏障的完整性,导致细胞死亡.
- 识别的分子通路与露易石暴露时观察到的相似.
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