使用斑马鱼作为模型揭示重金属对发育的毒性:叙述性回顾
Sanjib Manna1, Sayed Mohammed Firdous2
1Department of Pharmacology, Calcutta Institute of Pharmaceutical Technology & AHS, Uluberia, Howrah, West Bengal, 711316, India.
概括
被重金属和微塑料暴露的斑马鱼胚胎显示出发育性毒性,包括形和改变基因表达. 这些发现突出了水生生态系统的风险和通过食物链对人类健康的潜在影响.
科学领域:
- 环境毒理学环境毒理学
- 发展生物学 发展生物学
- 生态毒理学 生态毒理学
背景情况:
- 发育性毒性会破坏生物体的正常发育.
- 斑马鱼 (Danio rerio) 是有价值的脊椎动物模型,因为它们快速繁殖,透明的胚胎和与人类的遗传相似性.
- 了解水生模型中的化学毒性对于环境和人类健康评估至关重要.
研究的目的:
- 研究斑马鱼胚胎中重金属和微塑料的发育毒性.
- 评估单次和同时暴露于各种重金属和微塑料的环境相关水平的影响.
- 确定这些污染物暴露在斑马鱼中的特定毒理终点和机制.
主要方法:
- 斑马鱼胚胎暴露于单个和组合的重金属 (,,,,,,,,,,,,铁,,,) 和微塑料.
- 观察和分析发育阶段,器官发生和形态变化.
- 评估分子变化,包括mRNA表达,反应性氧物种 (ROS) 生成,抗氧化剂水平,神经递质功能和亡蛋白表达.
主要成果:
- 重金属和微塑料诱导斑马鱼胚胎显著的发育毒性.
- 观察到的效应包括ROS的增加,抗氧化剂表达的减少,神经递质释放的改变和apoptotic蛋白表达的变化.
- 观察到的特定形包括心周胀,心脏胀,长度减小,尾巴曲和异常形成.
- 与单次暴露相比,同时暴露改变了毒理结果.
结论:
- 斑马鱼是重金属和微塑料毒性的敏感指标.
- 这些污染物对水生生物的发展和生态系统健康构成重大风险.
- 由于食物链中的生物积累存在潜在的人类健康风险.
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