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在植物中通过3' UTR编辑进行基因表达的有条件淘汰

Tianzhen Liu¹, Jiaqi Shen¹, Dating Zhong²

¹Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Plant communications

|February 23, 2025

概括

基因组编辑通过将微RNA (miRNA) 点集成到基因中,使得植物中精确的基因敲除成为可能. 这种miRNA介导的,局部淘汰 (MiRKD) 方法允许控制基因表达,这对于作物分子育种至关重要.

科学领域:

植物分子生物学植物分子生物学
基因组编辑技术的技术.
农作物分子育种农作物分子育种

背景情况:

时间空间基因敲除是分子育种的一个关键目标.
内源微RNAs (miRNAs) 通过目标识别来调节基因表达.
现有的基因淘汰方法缺乏精确的时空控制.

研究的目的:

开发一种使用基因组编辑精确的时空基因抑制的新方法.
为了研究创造人工miRNA依赖的调节电路的可行性.
为了验证米饭中miRNA介导的局部敲除 (MiRKD) 的有效性.

主要方法:

对米米RNA表达特征进行分析,以选择内源的miRNAs.
通过基因组编辑将miRNA目标序列集成到目标基因的3'未翻译区域 (UTR).
过渡性测试和稳定编辑的水植物的生成,以评估基因表达水平.
利用GID1作为标基因来评估不同的miRNA敲入.

主要成果:

在3' UTR中将miRNA点的局部整合有效地减少了目标基因的空间和时间表达.
针对miR156a的Knockin导致GID1表达的97%的组成性减少.
针对miR396c的诺基因特异性降低了GID1在射击中的表达.
在长日条件下,miR528目标的Knockin导致GID1表达的时间下降95%.

结论:

通过MiRNA介导的局部淘汰 (MiRKD) 是一种可行且方便的作物育种方法.
这种方法可以通过利用内源miRNA表达模式来进行条件基因抑制.
基因组编辑与miRNA向相结合,可以精确控制植物中的基因功能.

关键词:

有条件的敲击下降.农作物育种植物育种基因组编辑基因组编辑这是一个小RNARNA.米米饭米饭米饭米饭.

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