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对比单细胞分析揭示了肌原始器功能障碍由年龄相关的氧化应激和其恢复通过抗氧化剂治疗
Youngjae Jeong1, Dongwook Yang1, Jea Giezl Solidum1,2
1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
Journal of cellular physiology
|February 23, 2025
概括
成人的肌显示炎症和氧化应激增加,损害愈合. 抗氧化剂治疗可以减轻这些与年龄相关的变化,改善肌的修复和功能.
科学领域:
- 生物医学科学 生物医学科学
- 再生医学是一种再生医学.
- 肌肉骨生物学 肌肉骨生物学
背景情况:
- 成人的肌愈合通常因纤维化而受损,与新生儿肌完全恢复功能不同.
- 肌细胞和肌干细胞/原生细胞 (TSPC) 中与年龄相关的细胞和分子变化,导致愈合受损,尚不清楚.
研究的目的:
- 研究出生后和成年小鼠肌细胞和TSPCs之间的与年龄相关的细胞和分子差异.
- 确定成年人肌愈合障碍背后的机制,并探索潜在的治疗干预措施.
主要方法:
- 早期产后 (2周) 和成年 (20周) 鼠标肌的比较单细胞转录学分析.
- 在体内评估成年肌细胞中细胞内活性氧物种 (ROS) 水平.
- 评估抗氧化剂治疗对成年小鼠ROS水平和肌强度的影响.
主要成果:
- 成人的肌表现出减少的TSPC群体,与肌和软骨发育相关的基因表达减少,以及增加的纤维肌细胞群体.
- 成人TSPC和细胞显示免疫反应和氧化应激基因的表达增加,EGFR升高,IGF信号减少.
- 在体内成人肌细胞中观察到细胞内ROS的增加,抗氧化剂治疗可以减少这种增加,从而改善肌强度.
结论:
- 炎症增加和ROS有助于肌衰老,损害功能和再生.
- 抗氧化剂治疗可以减轻与年龄相关的ROS积累和成人肌炎症.
- 这些发现突出了改善老年人肌愈合和功能的潜在治疗策略.
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