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在生物体内发育的牛卵细胞和植入前胚胎中的多种A选择性基因间转录.

Saurav Ranjitkar1, Mohammad Shiri2, Jiangwen Sun2

  • 1Department of Animal Science, University of Connecticut, Storrs, Connecticut, USA.

Molecular reproduction and development
|February 23, 2025
PubMed
概括
此摘要是机器生成的。

在体内发育的牛胚胎和卵细胞表现出丰富的基因间转录,称为读出和读入,读透转录有限. 这些转录与发育阶段和基因表达相关,独立于DNA甲基化模式.

关键词:
跨基因转录 跨基因转录聚亚脱化 聚亚脱化 聚亚脱化在阅读中读入.读出来的读出方式读透 (基因的下游)

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科学领域:

  • 发展生物学 发展生物学
  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 基因间转录,包括读透转录和基因间区域的转录启动,在培养细胞中观察到,可以通过压力增强.
  • 这种现象在自然生物样本中并没有得到很好的描述,特别是在植入前的发育过程中,这一时期被广泛的基因表达和DNA甲基化变化所标志.

研究的目的:

  • 在牛卵细胞和植入前胚胎发育过程中体内表征基因间转录.
  • 在这些早期发育阶段调查基因间转录,基因表达和DNA甲基化之间的关系.

主要方法:

  • 使用自动读透转录检测 (ARTDeco) 和在体内发育的牛卵细胞和胚胎上进行多种A选择的RNA测序 (RNA-seq).
  • 分析了基因间转录类型 (读出,读入,读透),它们与参考基因的关联,差异表达和与DNA甲基化相关联.

主要成果:

  • 发现了大量的基因间转录,称为读出和读入,读透是不太常见的.
  • 许多基因间转录与跨发育阶段的共同参考基因有关,并显示了特定阶段的差异表达.
  • 尽管观察到甲基化下降,但在侧边区域的基因间转录和DNA甲基化水平之间没有发现显著的相关性.

结论:

  • 牛卵细胞和植入前胚胎表达大量的基因间转录,包括新的读出和读入.
  • 基因间转录不是随机的,与发育阶段和基因表达有关,并且似乎独立于广泛的DNA甲基化变化而受到调节.
  • 转录因子结合基因和多基化信号的存在表明,在基因间区域内,新型转录启动和RNA处理的活性调节.