在生物体内发育的牛卵细胞和植入前胚胎中的多种A选择性基因间转录
Saurav Ranjitkar1, Mohammad Shiri2, Jiangwen Sun2
1Department of Animal Science, University of Connecticut, Storrs, Connecticut, USA.
Molecular reproduction and development
|February 23, 2025
概括
在体内发育的牛胚胎和卵细胞表现出丰富的基因间转录,称为读出和读入,读透转录有限. 这些转录与发育阶段和基因表达相关,独立于DNA甲基化模式.
科学领域:
- 发展生物学 发展生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 基因间转录,包括读透转录和基因间区域的转录启动,在培养细胞中观察到,可以通过压力增强.
- 这种现象在自然生物样本中并没有得到很好的描述,特别是在植入前的发育过程中,这一时期被广泛的基因表达和DNA甲基化变化所标志.
研究的目的:
- 在牛卵细胞和植入前胚胎发育过程中体内表征基因间转录.
- 在这些早期发育阶段调查基因间转录,基因表达和DNA甲基化之间的关系.
主要方法:
- 使用自动读透转录检测 (ARTDeco) 和在体内发育的牛卵细胞和胚胎上进行多种A选择的RNA测序 (RNA-seq).
- 分析了基因间转录类型 (读出,读入,读透),它们与参考基因的关联,差异表达和与DNA甲基化相关联.
主要成果:
- 发现了大量的基因间转录,称为读出和读入,读透是不太常见的.
- 许多基因间转录与跨发育阶段的共同参考基因有关,并显示了特定阶段的差异表达.
- 尽管观察到甲基化下降,但在侧边区域的基因间转录和DNA甲基化水平之间没有发现显著的相关性.
结论:
- 牛卵细胞和植入前胚胎表达大量的基因间转录,包括新的读出和读入.
- 基因间转录不是随机的,与发育阶段和基因表达有关,并且似乎独立于广泛的DNA甲基化变化而受到调节.
- 转录因子结合基因和多基化信号的存在表明,在基因间区域内,新型转录启动和RNA处理的活性调节.
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01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
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