马莱克是一种内细胞网膜内定性莱克,促进了人类肝细胞癌的恶性行为
Dong Yu1, Fu Ming-Fen2, Liu Shao-Min1
1Department of oncology, The First Affiliated Hospital of Jinan University, No. 613, Huangpu Avenue West, Tianhe District, Guangzhou 510630, China.
Glycobiology
|February 23, 2025
概括
参与细胞内网膜应激的蛋白质马莱克丁在肝癌中被上调. 它的缺失阻碍了瘤的生长,迁移和入侵,这表明它在肝细胞癌的发展中发挥了作用.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
背景情况:
- 展开的蛋白质反应 (UPR) 提高了瘤细胞在细胞内网膜 (ER) 压力期间的存活率.
- Malectin 是一种 ER 常驻的莱克,有助于降解错误折叠的葡萄糖蛋白,并通过 ER 应力进行上调.
- 马莱克在癌症恶性瘤中的特定作用在很大程度上仍未被探索.
研究的目的:
- 调查人类肝细胞癌 (HCC) 中的马莱克的表达和功能.
- 确定雄性菌素对HCC细胞增殖,殖民地形成,迁移和入侵的贡献.
- 在体内评估马氏丁缺乏对HCC瘤生长的影响.
主要方法:
- 在人类HCC组织和细胞系中分析雄性素表达.
- 在HCC细胞系 (HepG2,QGY-7703) 中,CRISPR-Cas9介导的雄性素的淘汰.
- 在体外测试细胞增殖,殖民地形成,迁移和入侵.
- 在裸体小鼠异种移植模型中的体内瘤生长评估.
主要成果:
- 与正常对照组相比,HCC组织和细胞系中马列丁表达显著上调.
- 马莱克丁淘汰并没有影响HCC细胞的增殖,但显著减少了殖民地形成,迁移和入侵.
- 在裸体小鼠中植入时,马莱克缺乏的HCC细胞表现出显著下降的瘤生长.
结论:
- 马莱克丁在肝细胞癌中异常表达.
- 马莱克在促进HCC细胞迁移,入侵和瘤生长方面发挥着至关重要的作用.
- 马莱克丁可能代表HCC治疗的潜在治疗标.
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