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Updated: May 26, 2025

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
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Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization

Published on: April 5, 2019

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一种通过碳氧化物表面改性聚合物基质获得的超分支离子交换器,用于离子染色学

Yufeng Shen1, Xiaomei Zhu1, Feifang Zhang1

  • 1Engineering Research Center of Pharmaceutical Process Chemistry, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, China.

Journal of chromatography. A
|February 23, 2025
PubMed
概括

一种新型的碳氧化聚合物基质使得高分支离子交换染色学成为可能,为分析各种离子提供了高效率. 这种方法避免了硫酸盐出血,这对于纯水中微量离子分析至关重要.

关键词:
阳离子交换器的使用.协价结合是一种共价结合.静电固定装置的固定方式超分支 超分支的 超分支的离子染色学 离子染色学

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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation12:55

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Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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