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Updated: May 26, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

EasyCircR:检测和重建循环RNA的转录后调控相互作用网络

Antonino Aparo¹, Simone Avesani², Luca Parmigiani³

¹Department of Computer Science, University of Verona, Strada le Grazie, 15, Verona, 37134, Italy; Research Center LURM (Interdepartmental Laboratory of Medical Research), University of Verona, Verona, 37134, Italy.

Computers in biology and medicine

|February 23, 2025

概括

EasyCircR是一个新的R包,用于分析循环RNA (circRNA) 相互作用. 它准确地识别了circRNA-miRNA-mRNA网络,专注于转录后调节,以获得可靠的生物学见解.

科学领域:

分子生物学分子生物学
生物信息学是一种生物信息学.
基因组学就是基因组学.

背景情况:

循环RNAs (circRNAs) 是生物过程中的关键调节者,与神经系统疾病和癌症有关.
循环RNAs通过转录,拼接,翻译和翻译后修改影响基因表达.
一些circRNAs充当microRNA (miRNA) 的海绵,在转录后增强目标基因表达.

研究的目的:

开发一个新的R包,EasyCircR,用于全面的circRNA分析.
为了准确地重建circRNA-miRNA-mRNA调节网络.
通过circRNAs区分转录后基因调节,避免不准确的预测.

主要方法:

开发了EasyCircR,这是一个整合circRNA检测和重建的R包.
纳入的转录后基因表达分析 (外体内体分裂分析).
通过Shiny应用程序实现了miRNA响应元素预测和可视化.

主要成果:

易CircR可以准确地检测和重建circRNA.
该包有效地识别了circRNA-miRNA相互作用和海绵式miRNAs.
EasyCircR可视化复杂的circRNA-miRNA-mRNA网络,专注于转录后调节.

结论:

EasyCircR提供了一个强大的工具来分析circRNA介导的基因调节.
该包提高了circRNA-miRNA-mRNA网络重建的准确性.
EasyCircR促进了对生物系统中circRNA功能的更深入的理解.

关键词:

循环RNA 是一个循环RNA.循环RNA-miRNA-mRNA网络中的一个.这就是米RNA.转录后的监管转录后的监管它们是mRNARNA.

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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