普罗西亚尼丁B2通过抑制TLR4/NF-κB和caspase-3/GSDME通路来减轻甲基甲酸诱导的肝炎
Juman Alsaab1, Wedad S Sarawi1, Ahlam M Alhusaini1
1Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh, 11495, Saudi Arabia.
普罗基亚尼丁B2 (PCB2) 显示出对甲基酸 (MTX) 诱导的肝损伤的保护作用. PCB2表现出抗氧化,抗炎和抗火性质,减轻了老鼠的MTX毒性.
科学领域:
- 肝病学 肝病学是一种肝病学.
- 药理学 药理学是指药理学的学科.
- 毒理学 毒理学 毒理学
背景情况:
- 甲甲酸 (MTX) 是一种重要的化疗和免疫抑制药物.
- 即使在低剂量中,MTX诱导的肝毒性也是一个重要的临床问题.
- 了解防治MTX肝损伤的保护剂至关重要.
研究的目的:
- 调查普西亚尼丁B2 (PCB2) 对MTX诱导的肝毒性的潜在肝脏保护作用.
- 评估PCB2对MTX治疗大鼠的氧化应激,炎症和火灭菌标记物的影响.
主要方法:
- 鼠被治疗PCB2 (40毫克/公斤) 或氨酸 (阳性对照,20毫克/公斤),然后注射MTX (20毫克/公斤).
- 血清中氨基酶,肝氧化/抗氧化标志物 (MDA,GSH,SOD),炎症性细胞因子 (TNF-α,IL-1β,IL-6) 和关键蛋白表达 (NF-κB,TLR4,caspase-3,GSDME) 被分析.
主要成果:
- 服用MTX显著增加了血清中氨基酸酶,表明肝损伤.
- MTX诱导了肝氧化应激,增加了炎症性细胞因子,并上调了NF-κB,TLR4,caspase-3和GSDME.
- 通过恢复氧化剂/抗氧化剂平衡,减少炎症和热的标志物,PCB2治疗显著改善了MTX诱导的肝毒性.
结论:
- 普罗西安丁B2在大鼠中表现出显著的肝保护作用,防止甲状腺酸诱导的肝损伤.
- PCB2表现出抗氧化,抗炎和抗热的活性,这表明其潜在的治疗作用.
- 需要进一步的研究来证实PCB2作为MTX肝毒性保护疗法的有效性.
更多相关视频
08:49Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Published on: January 22, 2019
11:00Methods to Inhibit Bacterial Pyomelanin Production and Determine the Corresponding Increase in Sensitivity to Oxidative Stress
Published on: August 31, 2015
相关概念视频
Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents
Phase II Reactions: Miscellaneous Conjugation Reactions
A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
The Intrinsic Apoptotic Pathway
