"曼诺斯甘氨酸作为训练免疫力的关键参与者:一种新的抗瘤催化剂"
Pedro Almeida1, Inês Alves1, Ângela Fernandes1
1Institute for Research and Innovation in Health (i3S), University of Porto, 4200-135 Porto, Portugal.
Biochimica et biophysica acta. General subjects
|February 23, 2025
概括
酵母衍生的曼南诱导单细胞中的训练免疫 (TI),增强它们的炎症反应和抗瘤活性. 这种糖甘原始也改变了免疫细胞标记物,为癌症免疫治疗提供了潜在的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 葡萄糖生物学 葡萄糖生物学
- 癌症研究 癌症研究
背景情况:
- 众所周知,来自微生物的细胞壁甘氨酸可在像单细胞这样的先天免疫细胞中诱导训练免疫力 (TI).
- 酵母衍生的曼南在单细胞重编程和TI中的具体作用尚不清楚.
研究的目的:
- 调查酵母衍生的曼南对单细胞中训练免疫力的贡献.
- 为了确定以曼南为原料的单细胞是否表现出增强的抗瘤活性.
- 评估用BCG治疗的膀癌患者单细胞中的TI表型.
主要方法:
- 在单细胞中分析糖甘结合蛋白 (DC-SIGN,Dectin-2) 的基因表达特征.
- 使用曼南在健康单细胞中诱导TI.
- 评估促炎性细胞因子分泌 (TNFα,IL-6) 和CD86激活水平.
- 在体外对曼南训练的单细胞杀死瘤细胞的能力的评估.
- 在BCG治疗后的非肌肉侵入性膀癌患者的单细胞中分析TI表型.
主要成果:
- 曼南在单细胞中触发了增强的免疫表型,与TI一致.
- 曼南原型单细胞显示增加了促炎性细胞因子分泌和CD86表达.
- 曼南改变了糖甘结合蛋白的表达,包括DC-SIGN.
- 曼南训练的单细胞表现出改善的体外瘤细胞杀伤.
- 接受BCG治疗的膀癌患者的单细胞表现出TI表型.
结论:
- 酵母衍生的曼南可以诱导单细胞的训练免疫力,增强其免疫功能.
- 曼南原质单细胞显示出增加的抗瘤潜力.
- 这项研究为使用甘氨酸衍生病原体组件重新编程抗癌免疫疗法的先天性免疫细胞提供了基础.
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