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采用旋转内部流量层工程技术的半自动层次生物制造

Gwyneth West¹, Sneha Ravi², Jamie A Davies³

¹Deanery of Biomedical Science, The University of Edinburgh, Edinburgh, United Kingdom.

SLAS technology

|February 23, 2025

概括

自动化旋转内部流层工程 (RIFLE) 提高了人体组织生产规模和可重复性. 半自动化RIFLE与原凝显示高细胞活力,推进生物制造用于研究和临床使用.

科学领域:

生物技术是生物技术.
组织工程是组织工程.
再生医学是一种再生医学.

背景情况:

生物制造旨在扩大和提高人类组织生产的可再生性.
旋转内部流量层工程 (RIFLE) 创建微尺度的分层管状结构.
目前的RIFLE方法需要手动应用充满细胞的水凝.

研究的目的:

为了自动化RIFLE生物制造工艺的关键元素,特别是液体配送.
为了评估自动化RIFLE系统的性能,使用酸盐和原蛋白水凝.
评估自动化对工程组织细胞活力和层精度的影响.

主要方法:

为RIFLE.开发和实施自动化液体分配系统.
使用酸盐和原蛋白作为带有细胞的水凝材料.
在手动和半自动RIFLE过程中比较细胞活力和层形成.
在工程结构中表征细胞分层的分辨率.

主要成果:

半自动组装实现了与手工方法相比的细胞生存率.
自动化原体水凝在10天内显示出高细胞活力 (>91%).
半自动化的RIFLE系统精确地将细胞种群组装成细胞宽度层 (≈14微米).
自动化减少了手动操作和人为错误的可能性.

结论:

自动化RIFLE过程提高了生物制造中的效率和可重复性.
在使用RIFLE的自动化组织工程应用中,原体水凝是有利的.
为了更广泛的生物制造进步,建议进一步自动化,特别是在细胞-凝悬浮制剂中.

关键词:

阿尔金酸盐是一种酸盐.自动化自动化自动化自动化自动化生物制造的生物制造原蛋白是一种原蛋白.射手射手射手射手射手组织工程是组织工程.管状组织管状组织

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