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含有微生物核酸的基于脂质的纳米颗粒的局部输送用于骨免疫调节

N R Rahmani¹, F Jahanmard², A Hassani Najafabadi³

¹Department of Orthopedics, University Medical Center Utrecht, Heidelberglaan 100, GA 3508, Utrecht, the Netherlands; Regenerative Medicine Center Utrecht, University Utrecht, Heidelberglaan 8, CS 3584, Utrecht, the Netherlands.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

|February 23, 2025

概括

在纳米颗粒中封装的CpG ODN C通过调节免疫细胞显示了骨再生的潜力. 然而,由于BMP-2的度不足和潜在的掩盖效应,目前的体内结果是不确定的.

科学领域:

生物材料科学生物材料科学
免疫学免疫学免疫学
再生医学是一种再生医学.

背景情况:

骨免疫调节通过操纵免疫微环境来增强骨再生.
含有CpG的Oligonucleotides类型C (CpG ODN C) 和Polyinosinic:polycytidylic acid (Poly[I:C]) 是微生物核酸类似物,具有免疫治疗潜力.

研究的目的:

调查CpG ODN C和Poly (I:C) 作为骨质免疫调节剂用于骨再生.
评估基于脂质的纳米颗粒的有效性,为骨植入物应用提供这些核酸.

主要方法:

CpG ODN C和Poly ((I:C) 被封装在基于脂质的纳米粒子中 (Lip/CpG ODN C,Lip/Poly[I:C]).
评估了对巨细胞因子 (TNF,IL-6,IL-10) 和骨质细胞分化的影响.
含有支架,BMP-2和GelMA水凝中的纳米颗粒的骨植入物在子外骨架模型中进行了5周的测试.

主要成果:

与自由核酸相比,Lip/CpG ODN C和Lip/Poly[I:C]在巨细胞中增强了细胞因子的产生.
在实验室中,CpG ODN C和Lip/CpG ODN C促进了骨质细胞分化.
在体内,所有植入组都表现出骨形成,但新骨体积和支架退化在各组中都相似.

结论:

唇/CpG ODN C 在体外显示了骨质免疫调节的潜力.
不足够的核酸度和潜在的BMP-2掩盖效应可能会限制体内结果.
需要进一步的体内研究来证实Lip/CpG ODN C在骨再生方面的骨免疫调节潜力.

关键词:

骨再生医学是一种骨再生医学.在生物体内,在生物体内.骨质免疫调节骨质免疫调节这就是PAMP的作用.病原体识别受体的病原体识别受体收费类受体的收费类受体

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