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来自Panax notoginseng的PnNAC03在细胞壁形成过程中积极调节沙素和素生物合成的功能

Xiaoqin Zhang^1,2, Yuying Huang^1,3, Yue Shi¹

¹School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, People's Republic of China.

Plant cell reports

|February 23, 2025

概括

PnNAC03增强了Panax中二次细胞壁中的氨酸和氨酸的产量. 这种转录因子直接激活关键基因,对药用植物的发展和抗压能力至关重要.

科学领域:

植物分子生物学植物分子生物学
生物化学生化学
植物生理学植物生理学

背景情况:

NAC转录因子调节二次代谢物生物合成.
沙素是药用植物Panax notoginseng中的关键生物活性化合物.
二级细胞壁对于植物生长和抗压能力至关重要.

研究的目的:

描述PnNAC03在调节沙素生物合成和二次细胞壁形成中的作用.
为了研究PnNAC03对目标基因的作用的分子机制.
为了探索PnNAC03在Panax notoginseng分子育种中的潜力.

主要方法:

在Panax notoginseng calli.中用于基因沉默的RNA干扰 (RNAi).
对氨酸和氨酸生物合成基因的基因表达分析 (qRT-PCR).
促进体结合测定证实了直接的转录调节.
在Arabidopsis thaliana进行过度表达研究以验证功能.

主要成果:

制PnNAC03显著降低了沙素含量和关键沙素基因 (PnSS,PNSE,PNDS) 的表达.
PnNAC03直接结合了氨酸和氨酸生物合成基因 (PAL,C4H) 的促进剂,增强了它们的表达.
过度表达PnNAC03增加了Arabidopsis中的二次细胞壁厚度和素含量,上调了AtPAL和AtC4H.

结论:

PnNAC03作为转录激活剂,积极调节氨酸生物合成和氨酸积累.
在二次细胞壁形成中,PnNAC03的双重作用是由直接结合目标基因促进体的介导.
通过分子育种,PnNAC03是增强Panax notoginseng药用特性和抗压能力的有价值的目标.

关键词:

帕纳克斯并没有oginsengeng.在Lignin的基础上.在NAC转录因子的转录因子.萨宁的生物合成二次细胞壁中的二次细胞壁.

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