相关实验视频

Updated: May 26, 2025

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

经过修改的散装分离分析,在芽酵母中确定了表观性和神秘的QTL对铜抵抗的分析

Cassandra Buzby^1,2, Yevgeniy Plavskin^1,2, Federica M O Sartori^1,2

¹Department of Biology, New York University, New York, NY 10003, USA.

|February 24, 2025

概括

我们开发了一种检测基因相互作用的新方法,称为史诗QTL分析,它改善了对酵母菌天然特征变异的研究. 这种强大的方法确定了七个影响铜电阻的相互作用位置.

科学领域:

遗传学遗传学是一种遗传学.
酵母遗传学酵母遗传学
量化遗传学量化遗传学

背景情况:

由于实验方法不足,估计自然特征变异的遗传相互作用 (epistasis) 很困难.
大量分离分析 (BSA) 经常通过平均基因型组合来掩盖表观症.

研究的目的:

开发一种高功率的方法来表征和量化自然特征变异背后的表观症.
为了确定与Saccharomyces cerevisiae中的特定染色体相互作用的遗传位置.

主要方法:

工程酵母菌株用于与一个固定的父母染色体交叉并分离其他染色体.
应用于这些交叉的批量分离分析 (BSA),以确定定量特征位置 (QTL).
开发了史诗式QTL (表皮性与染色体QTL) 分析,以检测QTL和固定的染色体之间的相互作用.

主要成果:

在不同酵母菌株之间的交叉中,确定了7个与第八染色体相互作用的基位,用于抗铜.
没有检测到与染色体I的显著相互作用.
相互作用改变了附加的QTL效应的大小,而不是方向.
修复第八染色体增加了检测其他贡献位置的功率.

结论:

史诗-QTL分析为在自然种群中检测表现症提供了高的统计能力.
染色体VIII通过相互作用在调解抗铜性方面发挥着重要作用.
修复染色体变异可以提高对复杂特征的遗传结构的检测.

关键词:

这种植物是Saccharomyces cerevisiae.硫酸铜硫酸盐是什么意思史诗主义就是一种史诗主义.定量特征的位置 (loci)电阻的阻力可以酵母遗传学酵母遗传学

更多相关视频

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

相关概念视频

Epistasis Analysis

Epistasis Analysis

Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...

Epistasis

Epistasis

In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...