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Updated: Jul 23, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

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CnRed:通过适应的兰巴达红色重组组合,对Cupriavidus necator H16进行高效的无标记基因组工程

Simon Arhar1,2, Johanna Pirchner1, Holly Stolterfoht-Stock1

  • 1Austrian Centre of Industrial Biotechnology, acib GmbH, 8010 Graz, Austria.

ACS synthetic biology
|February 24, 2025
PubMed
概括

我们为Cupriavidus necator开发了一个新的基因组编辑系统,Cupriavidus necator是一种具有生物技术巨大潜力的细菌. 这种工具可以实现更快,更有效的基因改造,加速改进的生产菌株的开发.

关键词:
这就是Cupriavidus necator.电穿孔的电穿孔是什么基因工程是基因工程,是基因工程.兰达红色 兰达红色植物酶是一种植物酶.它们是重组组合的.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...