使用马便制备和储存便微生物群移植溶液后细菌活力的变化
Julia A Arantes1, Rebecca Di Pietro2, Mélanie Ratté2
1Department of Veterinary Medicine/Faculty of Animal Science and Food Engineering, Universidade de São Paulo, Pirassununga, São Paulo, Brazil.
PeerJ
|February 24, 2025
概括
对于马来说,便微生物群移植 (FMT) 受结的影响. 糖在冷后保持细菌活力方面优于其他保护剂,尽管新鲜的FMT可能是理想的.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 马的健康 马的健康
背景情况:
- 便微生物种移植 (FMT) 是一种治疗马的胃肠道疾病的方法.
- 在FMT制剂中优化细菌存活对于疗效至关重要.
- 目前正在研究马类FMT的准备和保护方案.
研究的目的:
- 评估氧气暴露和保护剂溶液对结前和后马便中的细菌活力的影响.
- 为了比较抗氧化剂冷保护剂溶液与10%甘油在保护细菌活力的有效性.
- 评估冷解对马便样本中的细菌活力的影响.
主要方法:
- 从10匹健康的马匹收集了马便样本.
- 样品被稀释在抗氧化剂溶液或10%的甘油中.
- 样品是在无氧或环境空气条件下准备的,并在新鲜和冷后 (-20°C90天) 进行分析.
- 使用流式细胞计量评估了细菌活力.
主要成果:
- 冷解显著降低了47%的细菌活力.
- 糖醇 (32 ± 8%) 在冷后保持生命力方面明显比抗氧化剂冷保护剂 (24 ± 8%) 更有效.
- 暴露于氧气并没有显著影响细菌活力 (p = 0.13).
结论:
- 新鲜的FMT溶液可能更适合用于治疗马匹失生症.
- 如果冷是必要的马类FMT制备,糖醇是推的稀释剂,以保持细菌活力.
- 适当的样本处理和冷保存对于保持马匹FMT的治疗潜力至关重要.
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