数据集用于对精密元基因组学和尿路感染诊断传统方法进行比较分析
Rob E Carpenter1,2, Sadia Almas3, Vaibhav K Tamrakar4
1Department of Research, Advanta Genetics, Tyler, TX 75703, United States.
Data in brief
|February 24, 2025
概括
与传统方法相比,精密元基因组学为尿路感染 (UTI) 提供了更高的诊断产量. 这种先进的测序识别了更多的尿病原体,包括多微生物感染,提高了尿路感染的诊断和管理.
科学领域:
- 临床微生物学 临床微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 微生物培养是诊断尿路感染的黄金标准,但在敏感度上有局限性.
- 聚合酶链反应 (PCR) 提供更高的灵敏度,但仅限于预先准的生物.
- 精密元基因组学为病原体识别提供了一个广泛的,目标不可知的方法.
研究的目的:
- 为了比较微生物培养,PCR和精密元基因组学的诊断性能,用于检测尿样中的尿病原体.
- 评估精密元基因组学能够识别更广泛的病原体谱的能力,包括传统方法错过的病原体.
- 为未来的尿道感染微生物组和诊断研究提供一个全面的数据集.
主要方法:
- 通过微生物培养,PCR和精确的元基因组学,分析了47个怀疑患有尿路感染的患者的尿样.
- 通过使用Explify®平台使用精密元基因组学识别的病原体的生物信息分类.
- 在这三种方法中对病原体检测率的比较分析.
主要成果:
- 与PCR (19) 和微生物培养 (13) 相比,精密元基因组学发现了显著更多的尿病原体 (62).
- 98%的样本在精密元基因组学分析时显示出多微生物感染.
- 精确的元基因组学检测到通过培养和PCR错过的病原体,包括培养负和PCR负的病例.
结论:
- 精确的元基因组学证明了尿路感染检测的优越诊断产量.
- 这种方法对于识别多微生物感染和罕见病原体至关重要,促进了尿路感染诊断.
- 生成的数据集支持进一步研究尿路感染微生物群,病原体发现和抗菌素耐药性.
相关概念视频
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Microbiota of the Urogenital Tract
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Urine Studies II: Urine Culture and Sensitivity Test
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care
A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...


