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Updated: May 26, 2025

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哺乳动物卵细胞从颗粒细胞中接收母性效应RNA

Caroline A Doherty^1,2, Abdulfatai Tijjani³, Steven C Munger^3,4

¹Department of Obstetrics, Gynecology and Reproductive Medicine; San Francisco, CA, 94143.

bioRxiv : the preprint server for biology

|February 24, 2025

概括

生长中的卵细胞从周围的粒状细胞中接收到必需的信使RNA (mRNA),而不仅仅是小分子. 这一发现挑战了以前关于卵细胞发育过程中营养和分子转移的假设.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

哺乳动物卵细胞被认为只能通过间隙结接从颗粒细胞中接收小分子.
支持卵细胞生长的分子交换的精确机制仍然不完全理解.

研究的目的:

为了调查成长中的哺乳动物卵细胞是否会从周围的粒状细胞中接收更大的分子,特别是信使RNA (mRNA).
为了确定卵细胞内的颗粒素细胞衍生的mRNA类型,并探索它们在卵细胞发育中的潜在作用.

主要方法:

在小鼠中使用了嵌合体前腔卵细胞和花粉素细胞的重聚模型.
采用RNA测序来识别来自颗粒细胞的卵细胞内的转录.
进行免疫染色以可视化RNA结合蛋白 (FMRP和TDP43) 的定位及其与细胞结构的关联.

主要成果:

在成长中的小鼠卵细胞中鉴定出超过1000个颗粒素转录RNA.
发现颗粒素衍生转录的丰富对于卵细胞成熟和早期胚胎发育至关重要.
观察到RNA贩运蛋白质FMRP和TDP43与与卵细胞连接的富含actin的颗粒状细胞突起的同位.

结论:

生长中的卵子细胞积极从伴侣颗粒状细胞中进口mRNA,挑战了仅用于小分子转移的范式.
RNA结合蛋白FMRP和TDP43可能在贩运这些必要的母体转录物中发挥作用.
卵细胞发育失败可能源于母体转录物供应不足的粒状细胞,而不是仅仅是卵细胞内在缺陷.

关键词:

生育能力生育能力生育能力颗粒素细胞是颗粒素的细胞.卵细胞是卵细胞中的一个.转移RNA转移RNA是什么意思跨区域预测跨区域预测

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