Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Author Correction: Label-free human skin imaging with enhanced molecular contrast via time-resolved fluorescence and advanced phasor analysis.

Communications biology·2026
Same author

N-acetylcysteine modulates neutrophil-driven immune and metabolic pathways in steatotic liver ischemia-reperfusion injury.

Frontiers in immunology·2026
Same author

Collagen remodeling in murine melanoma therapy response through second-harmonic generation imaging.

Biophotonics discovery·2026
Same author

Label-free human skin imaging with enhanced molecular contrast via time-resolved fluorescence and advanced phasor analysis.

Communications biology·2025
Same author

In vivo human skin imaging: Distinguishing cellular and fibrillar components with fluorescence lifetime microscopy.

The Journal of investigative dermatology·2025
Same author

Quantum-enhanced detection of viral cDNA via luminescence resonance energy transfer using upconversion and gold nanoparticles.

Nanophotonics (Berlin, Germany)·2025

相关实验视频

Updated: May 26, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

11.9K

GSLab:在光显微镜中进行高级相位分析的开源平台.

Alexander Vallmitjana1, Belén Torrado1, Amanda F Durkin1

  • 1Beckman Laser Institute and Medical Clinic, University of California, Irvine.

bioRxiv : the preprint server for biology
|February 24, 2025
PubMed
概括
此摘要是机器生成的。

GSLab提供了一个用于光显微镜图像分析的开源平台,通过机器学习和定量脱混合来增强相位分析. 这种工具有助于研究人员解决复杂的生物问题.

更多相关视频

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

795
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.4K

相关实验视频

Last Updated: May 26, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy
09:30

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

11.9K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

795
From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

11.4K

科学领域:

  • 生物物理学的生物物理.
  • 显微镜的使用方法
  • 计算生物学 计算生物学

背景情况:

  • 有效的图像分析对于光显微镜至关重要.
  • 传统的分相分析在复杂的生物样本中存在局限性.
  • 需要在光成像中使用先进,易于使用的工具.

研究的目的:

  • 推出GSLab,这是一个开源平台,用于在光显微镜中进行先进的相位分析.
  • 通过机器学习和定量脱杂来增强传统的相位分析.
  • 为研究人员提供全面的工具来应对复杂的生物成像挑战.

主要方法:

  • 开发一个开源软件平台,GSLab.
  • 集成基于机器学习的分类以获取相位数据.
  • 实时监控和定量脱杂算法的实施.
  • 与商业和定制光显微镜系统的兼容性.

主要成果:

  • GSLab通过先进的计算工具增强了传统的相位分析.
  • 该平台允许基于机器学习的光寿命和光谱数据的聚类.
  • 光物种的定量分离是通过GSLab的功能实现的.
  • 实时监控功能改善了实验工作流程.

结论:

  • GSLab提供了一个强大的开源解决方案,用于光显微镜图像分析.
  • 该平台使研究人员能够使用先进的相位分析来解决复杂的生物学问题.
  • 通过增强的成像技术,GSLab促进了对生物系统的更深入的洞察.