相关实验视频

Updated: May 26, 2025

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

在Piezo1上,TRPV4主导了高剪切诱导的初始引反应和长期放松

Mohanish K Chandurkar^1,2, Manli Yang³, Majid Rostami^1,2

¹Department of Biomedical Engineering, Michigan Technological University, Houghton, Michigan, United States.

bioRxiv : the preprint server for biology

|February 24, 2025

概括

内皮细胞通过Piezo1和TRPV4离子通道对流体剪切应力 (FSS) 作出反应. TRPV4在最初的引变化和长期放松中起着关键作用,对血管健康和动脉样硬化至关重要.

科学领域:

内皮细胞机械生物学
血管生理学血管生理学
离子通道功能离子通道功能

背景情况:

内皮细胞对流体剪切应激 (FSS) 的反应对血管健康和动脉样硬化至关重要.
之前的研究表明,在高和低FSS下,有明显的引力调节.
这些FSS诱导的引反应中介的上游机械传感器以前是未知的.

研究的目的:

研究Piezo1和TRPV4离子通道在FSS下调节内皮细胞引力的作用.
阐明这些离子通道对内皮细胞机械传导的独特贡献.

主要方法:

使用了人类静脉内皮细胞 (HUVECs),具有沉默的Piezo1 (siPiezo1) 和TRPV4 (siTRPV4) 表达.
在不同的FSS条件下 (高和低) 测量了内皮细胞的引力.
分析了FSS暴露后的引力大小,对齐和时间动态.

主要成果:

siPiezo1在高FSS下部分降低了初始引力的增加,但没有影响低FSS反应或对齐.
siTRPV4完全取消了在高和低FSS下的初始引升和对齐.
双抑制Piezo1和TRPV4损害了初始和长期的引力,显著增加了低FSS反应,表明了其他途径.

结论:

皮埃佐1和TRPV4都是响应FSS的内皮细胞的关键机械传感器.
与Piezo1相比,TRPV4在调解最初的引反应和长期放松方面起着更为主导的作用.
这些发现凸显了Piezo1和TRPV4对内皮细胞机械转导和血管重塑的独特和关键贡献.

关键词:

内皮细胞内皮细胞流动机械传导是流动的机制传导.流体剪切应力是流体剪切应力.焦点附着性焦点附着性这是一个Piezo1.在 TRPV4 中.引力引力引力

更多相关视频

Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer

Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer

Published on: May 7, 2020

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

相关概念视频

Mechanically-gated Ion Channels

Mechanically-gated Ion Channels

Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...

Tension Response at Adherens Junctions

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...

Muscle Stimulation Frequency

Muscle Stimulation Frequency

The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...