子宫疾病对产后奶牛卵细胞发育能力的短期和长期影响
bioRxiv : the preprint server for biology
|February 24, 2025
概括
乳牛的早期产后子宫疾病,特别是子宫内膜炎,会对卵细胞质量和胚胎发育产生负面影响. 对卵细胞分裂率的这种影响在产后一个月和可能长达80天内可观察到,但在产后165天内却无法观察到.
科学领域:
- 生殖生物学 生殖生物学
- 兽医医学 兽医医学 兽医医学
- 动物科学动物科学
背景情况:
- 产后子宫疾病是奶牛常见的问题,与降低生育能力有关.
- 子宫疾病对卵细胞发育能力的具体影响仍然不完全理解.
研究的目的:
- 调查早期产后子宫疾病降低卵细胞发育能力,导致奶牛生育能力下降的假设.
- 确定子宫疾病对卵细胞质量和体外胚胎发育的时间影响.
主要方法:
- 乳牛在产后7-10天被诊断患有甲状炎或被归类为健康的乳牛.
- 生殖通道是在分娩后大约1个月,80天或165天收集的.
- 卵细胞复合体 (COC) 被收集,成熟,体外受精,并培养到胚芽细胞阶段.
主要成果:
- 在产后1个月内发生的子宫内膜炎 (purulent uterine lumen) 与卵细胞分裂率的降低有关 (Exp. 1). 1). 一个 1). 一个 1).
- 产后4-5周出现子宫疾病的奶牛的卵细胞在产后80天出现了减少的裂变率,但在产后165天却没有 (Exp. 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2). 2).
- 子宫疾病主要影响卵细胞分裂率,对胚胎细胞的发育或胚胎产量没有显著影响.
结论:
- 产后早期的子宫疾病,特别是子宫内膜炎,会损害卵细胞质量,由减少裂变率证明.
- 子宫疾病对卵细胞发育能力的负面影响存在于产后1个月和可能80天,但产后165天减少.
- 循环中的代谢物和子宫细菌的丰富性并不能显著解释体外卵子细胞发育的变化.
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