特定肠道区域的康门斯酸性酸化产生了对肠病原细菌感染的保护优先效应
bioRxiv : the preprint server for biology
|February 24, 2025
概括
一种来自野生的Lactiplantibacillus plantarum菌株通过降低肠道pH值来保护Drosophila melanogaster免受Serratia marcescens感染. 这种酸化抑制了病原体的生长,表明通过稳定的肠道微生物群相互作用介导的保护优先效应.
科学领域:
- 微生物学 微生物学
- 宿主-微生物相互作用
- 昆虫病理学 昆虫病理学
背景情况:
- 交叉体微生物通过优先效应提供对肠道病原体的保护.
- 机制包括抗微生物化合物,营养素竞争和pH值变化.
- 之前的研究使用了Drosophila melanogaster中不稳定的肠道殖民性的非本地Lactiplantibacillus plantarum菌株.
研究的目的:
- 通过自然发生的,稳定的肠道微生物关系来研究优先效应.
- 描述一种来自野生的乳植物菌株 (Lactiplantibacillus plantarum) 菌株对Serratia marcescens的保护机制.
主要方法:
- 隔离了一种野生衍生的Lactiplantibacillus plantarum菌株,用于稳定的肠道殖民.
- 在口服塞拉蒂亚马塞森斯感染后评估的存率和病原体负载.
- 进行了体外生长抑制测定和体内肠道成像,使用pH指标染料.
主要成果:
- 被野生类型L. plantarum殖民的对S. marcescens感染的抵抗力增加,寿命延长和病原体负载减少.
- 在体外实验中发现L. plantarum通过酸化抑制S. marcescens的生长.
- 在体内肠道成像表明,L. plantarum降低了肠道pH值,限制了S. marcescens的生长,并导致肠道中的空间分离.
结论:
- 来自野生的L. plantarum菌株建立了稳定的肠道殖民,并提供了对Drosophila melanogaster中的S. marcescens的保护.
- 由L. plantarum引起的肠酸化是这种保护优先效应的关键机制.
- 这项研究强调了使用自然存在的微生物关系来理解宿主微生物群相互作用和疾病耐药性的重要性.
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