Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

16.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.5K
CRISPR01:59

CRISPR

49.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.0K
Homologous Recombination02:31

Homologous Recombination

50.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structural and kinetic insights into a metagenomics-derived Cas12a with high specificity.

bioRxiv : the preprint server for biology·2026
Same author

Phage-encoded factor stimulates DNA degradation by the Hna anti-phage defense system.

Nature communications·2026
Same author

Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.

Nature reviews. Molecular cell biology·2026
Same author

Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency.

Nature structural & molecular biology·2026
Same author

Architecture of a DNA-guided Cas12a.

bioRxiv : the preprint server for biology·2026
Same author

Phage-encoded factor stimulates DNA degradation by the Hna anti-phage defense system.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: May 26, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.6K

二重功能II-B型CRISPR-Cas9的结构基础

Grace N Hibshman, David W Taylor

    bioRxiv : the preprint server for biology
    |February 24, 2025
    PubMed
    概括
    此摘要是机器生成的。

    来自Francisella novicida (FnCas9) 的Cas9使用REC3用于高保真基因组编辑. 这种独特的特性还使转录抑制成为可能,在生物技术和治疗中提供了双重应用.

    更多相关视频

    Substrate Generation for Endonucleases of CRISPR/Cas Systems
    11:53

    Substrate Generation for Endonucleases of CRISPR/Cas Systems

    Published on: September 8, 2012

    27.3K
    A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
    08:20

    A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

    Published on: September 2, 2021

    4.1K

    相关实验视频

    Last Updated: May 26, 2025

    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
    10:46

    Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

    Published on: October 18, 2022

    1.6K
    Substrate Generation for Endonucleases of CRISPR/Cas Systems
    11:53

    Substrate Generation for Endonucleases of CRISPR/Cas Systems

    Published on: September 8, 2012

    27.3K
    A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
    08:20

    A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

    Published on: September 2, 2021

    4.1K

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 遗传学 是一个
    • 生物技术是生物技术.

    背景情况:

    • 链球菌 pyogenes Cas9 (SpCas9) 酶被广泛用于基因组编辑,但由于对DNA-RNA不匹配的耐受性,可以导致非目标编辑.
    • 高准确性基因组编辑工具对于研究和治疗应用中精确的基因修改至关重要.

    研究的目的:

    • 调查来自Francisella novicida (FnCas9) 的Cas9的高保真DNA向的结构基础.
    • 探索FnCas9在DNA裂变和转录抑制中的双重功能.
    • 评估FnCas9在开发先进的基因组编辑技术和抗菌策略方面的潜力.

    主要方法:

    • 对FnCas9.9的动力和结构分析.
    • 生物化学测试以确定DNA准的准确性.
    • 进行X射线晶体学以阐明酶-RNA-DNA相互作用.
    • 使用小CRISPR关联RNA (scaRNA) 调查FnCas9介导的转录抑制.

    主要成果:

    • FnCas9拥有独特的REC3,这是一个结构特征,负责其内在的高保真DNA准.
    • REC3与R循环相互作用,建立一个检查点,在酶激活过程中增强特异性.
    • 在scaRNA的指导下,FnCas9可以抑制基因转录,这是其原生细菌环境中观察到的免疫逃避机制.
    • 结构数据揭示了部分R循环互补性如何防止裂变并有利于转录抑制.

    结论:

    • REC3是FnCas9高特异性的关键决定因素,并为精确的基因组编辑提供了一个新的机制.
    • FnCas9表现出双重作用模式,使DNA分裂和转录抑制成为可能,这对工程精确编辑器有意义.
    • REC3的保存性质表明,有可能开发新的抗菌策略,并提高基于CRISPR的治疗方法的精度.