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在瘤微环境中检测H2S的生物发光探头

Kang Lu¹, Yixian Wang¹, Chenhang Wang¹

¹Department of Chemistry, Rice University, 6100 Main Street, Houston, Texas 77005, United States.

ACS bio & med chem Au

|February 24, 2025

概括

研究人员开发了一种新的生物发光探头QTZ-N3,用于检测硫化物 (H2S) 在细胞癌 (RCC) 中. 这种探测器可以在没有外部刺激的情况下,对癌细胞和小鼠模型中的H2S水平进行敏感的实时监测.

科学领域:

生物化学生物化学
分子生物学分子生物学
医疗成像医学成像

背景情况:

硫化 (H2S) 是一个关键的信号分子,与各种疾病有关,包括细胞癌 (RCC).
目前用于RCC的H2S检测方法缺乏灵敏度,通常需要人工刺激,这限制了它们的体内适用性.
需要专门的,敏感的探测器来实时监测RCC进展中的H2S.

研究的目的:

开发一种新的H2S敏感生物发光探头,用于检测和监测细胞癌中的H2S.
评估探针在癌细胞中的灵敏度,选择性和有效性以及体内RCC模型.

主要方法:

用H2S识别组功能化的"子" luciferin 基质 (QTZ) 的设计和合成,创建了 QTZ-N3 探针.
使用生化分析评估探头灵敏度和选择性.
在786-O-Nluc RCC细胞和RCC异种移植裸体小鼠模型中验证QTZ-N3用于内源H2S检测.

主要成果:

QTZ-N3探头显示出良好的灵敏度 (∼0.19 μM) 和对H2S的选择性.
在没有外部刺激的情况下,QTZ-N3在RCC细胞中成功检测出内源的H2S.
在RCC异种移植裸体小鼠模型中实现了H2S水平的实时监测,与瘤进展相关.

结论:

QTZ-N3是一种敏感和选择性的生物发光探针,用于在RCC中检测H2S.
探测器可以在没有人工刺激的情况下,在RCC进展期间实时,体内监测H2S.
这项工作为了解H2S在RCC中的作用和开发新的诊断策略提供了有价值的工具.

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