在循环离子流动性质谱仪中进行多通道到达时间校正,用于成像和枪支脂管学
Pattipong Wisanpitayakorn1,2,3, Narumol Jariyasopit1,2,3, Kassaporn Duangkumpha1,2,3
1Siriraj Center of Research Excellence in Metabolomics and Systems Biology (SiCORE-MSB), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
ACS measurement science au
|February 24, 2025
概括
一种新方法纠正循环离子运动质谱 (cIM-MS) 漂移时间,改善质谱成像中的脂质识别和猎枪脂质组学. 这提高了不同实验室条件和仪器的准确性.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 质谱测量质量谱测量
背景情况:
- 直接输注质谱 (DI-MS) 和质谱成像 (MSI) 对脂质学至关重要,但由于缺乏分离,与异构和异构脂质注释作斗争.
- 循环离子流动性质谱 (cIM-MS) 提供了一个解决方案,但环境波动可能会影响离子流动性的到达时间,降低注释的可靠性.
研究的目的:
- 开发和验证cIM-MS的多通道到达时间校正方法,以提高脂质识别的准确性.
- 为了使cIM-MS数据可靠地在实验室间用于脂质注释,特别是MSI中的异构脂质.
主要方法:
- 在各种条件下 (日期,温度,设置,实验室) 使用参考标准开发了一种多通道到达时间校正方法.
- 用脂质和非脂质标准的混合物验证了该方法,并对人类和小鼠血清进行了枪支脂质学分析.
- 将新方法与MSI应用程序的碰撞截面确定进行了比较.
主要成果:
- 校正方法在各种实验条件中被证明是有效的,显著减少了平均漂移时间差异,从2%以上降至0.2%以下.
- 鉴定了脂质和非脂质分子之间的校正线略有变化,突出了需要适当的参考标准的需要.
- 在MSI中通过使用修正的多通道到达时间数据库进行单次测量,证明了成功的异构脂类鉴定.
结论:
- 开发的多通道到达时间校正方法提高了cIM-MS对脂管学的可靠性和准确性.
- 这种方法简化了MSI中的异构脂类鉴定,并且对环境变化和矩阵效应具有稳定性.
- 该方法促进了实验室间数据比较,并提高了对各种质谱技术脂质注释的信心.
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