银酸盐对性酸酶活性,TNF和IL-10基因表达的影响;实验和计算研究
Nader Jadid1, Ali Asghar Moshtaghie1, Hashem Nayeri1
1Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Heliyon
|February 24, 2025
概括
暴露于酸银会增加性酸酶 (ALP) 活性,并随着时间的推移升高瘤亡因子-α (TNF-α). 银酸盐治疗没有影响interleukin-10 (IL-10) 水平.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 毒理学 毒理学 毒理学
背景情况:
- 接触金属可以触发复杂的生化和免疫反应.
- 性酸酶 (ALP) 和炎症性细胞因子如IL-10和TNF-α是这些反应的关键指标.
研究的目的:
- 为了研究酸银对ALP活动的影响.
- 评估酸银对IL-10和TNF-α的调节作用.
主要方法:
- 与酶相关的免疫吸收试验 (ELISA) 用于细胞因子量化.
- 凝过色谱 (Sephacryl S300) 用于ALP分析.
- 用于ALP结合评估的分子对接.
主要成果:
- 酸银显著增加了高分子量ALP度.
- 在治疗后45天,TNF-α水平显著增加.
- 在对酸银的反应中,IL-10水平保持不变.
结论:
- 酸银调节ALP活动,有助于持续提升TNF-α.
- 这项研究强调了酸银的生化和免疫作用,特别是对TNF-α的影响.
相关概念视频
NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...


