尿道代谢概况受到常见储存条件和添加剂的最小影响
Kelly C Weldon1,2,3, Morgan Panitchpakdi2,3, Andrés Mauricio Caraballo-Rodríguez2,3
1School of Medicine, University of California Davis Health, Sacramento, CA, 95817, USA.
International urogynecology journal
|February 24, 2025
概括
尿液储存条件不会影响代谢分析. 虽然DNA稳定剂改变了代谢概况,但原代谢物仍然可以检测到,以准确地发现人类健康和疾病生物标志物.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 代谢学研究生物系统的通信.
- 尿液是一种有价值的,非侵入性的生物流体,用于识别健康和疾病生物标志物.
- 尿液储存和DNA稳定剂对新陈代谢的影响尚不清楚.
研究的目的:
- 调查常见的储存条件如何影响尿液代谢.
- 评估 DNA 稳定剂 AssayAssure® 对尿液代谢物质含量的影响.
主要方法:
- 尿样在各种储存条件下进行分析.
- 评估了AssayAssure®对尿中代谢物组成的影响.
- 代谢资料进行了比较,以评估变化.
主要成果:
- 在不同储存条件下,尿中代谢物概况一致.
- AssayAssure®的添加导致了通过 adduct 形成显著的代谢特征变化.
- 父代谢物识别和生物学差异的区分不受AssayAssure®的影响.
结论:
- 在测试条件下储存的尿液适合进行代谢分析.
- AssayAssure®不妨碍母代谢物检测,尽管改变了整体代谢特征.
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