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患有Bag5缺陷的男性生殖细胞显示精子生成和基本核蛋白的交换减少

Yuming Cao¹, Shengnan Wang¹, Zihan Qin^2,3

¹Department of Obstetrics and Gynecology, Perinatal Medical Center, The Fifth Affiliated Hospital of Sun Yat-Sen University, No. 52 Meihua East Road, Zhuhai, Guangdong, People's Republic of China.

Cellular and molecular life sciences : CMLS

|February 24, 2025

概括

与Bcl-2相关的乙基-5 (BAG5) 对男性生育能力至关重要,它调节精子发育和核蛋白沉积. 它的缺乏通过影响HSPA2表达和生殖细胞亡导致不孕.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
细胞生物学细胞生物学

背景情况:

与Bcl-2相关的乙基-5 (BAG5) 是一种参与调节伴侣活性的协伴蛋白.
BAG5在丸中表达高,对精子生成至关重要.
改变BAG5表达与男性不孕症有关,但潜在的机制尚不清楚.

研究的目的:

研究BAG5在男性生育中的作用及其分子机制.
探索BAG5,HSPA2和精子生成之间的关系.
评估BAG5作为男性不孕不育的潜在生物标志物.

主要方法:

精子和丸样本的RNA测序分析来自健康和不育的个体.
在小鼠中对Bag5的向基因失活.
免疫沉质谱 (IP-MS) 用于识别蛋白质相互作用.
评估生殖细胞的亡,核蛋白沉积和染色质凝结.

主要成果:

在小鼠中,Bag5的失活导致了生殖细胞的亡,不孕症,核蛋白负载的改变和精子头部的变形.
BAG5与HSPA2相互作用,HSPA2是精子生成和过渡蛋白 (TNPs) 的关键调节者.
Bag5 缺陷降低了HSPA2 水平,破坏了染色质凝结,并与非阻塞性亚精和橄酸精相关.

结论:

BAG5在男性生殖细胞发育和染色体组织中起着关键的HSPA2介导作用.
BAG5 缺乏导致通过胚胎细胞亡和核蛋白沉积受损的男性不孕症.
BAG5可以作为男性不孕症的预后生物标志物.

关键词:

BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 BAG5 不适当的核蛋白沉积不孕不育不孕不育不孕不育精子头的变形精子头的变形

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