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确定 lysosomal AMPK 激活的方法

Chen-Song Zhang¹, Sheng-Cai Lin²

¹State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, China.

Methods in molecular biology (Clifton, N.J.)

|February 24, 2025

概括

AMP激活蛋白激酶 (AMPK) 可以通过感知低葡萄糖代谢物果糖-1,6-双酸盐 (FBP) 的阿尔多酶在溶酶上独立于AMP/ADP被激活. 这条由甲和曼诺斯共享的途径涉及TRPV通道和v-ATPase.

科学领域:

细胞的新陈代谢
分子生物学分子生物学
生物化学生物化学

背景情况:

AMP激活蛋白激酶 (AMPK) 对于细胞能量恒温至关重要.
佳能AMPK激活依赖于AMP和ADP的绑定.
一个新的,非正规的AMPK激活途径已在 lysosome 表面被确定.

研究的目的:

详细介绍用于识别 lysosomal AMPK 激活的实验方法.
为了区分 lysosomal AMPK 激活与正规途径.
阐明触发 lysosomal AMPK 激活的分子参与者和条件.

主要方法:

评估AXIN lysosomal转位的情况.
测量 AMP:ATP 和 ADP:ATP 的比率.
确定 lysosomal pH 和 TRPV 通道活性.
使用葡萄糖代谢物果糖-1,6-双酸盐 (FBP) 作为传感器.

主要成果:

溶解体AMPK激活是独立于AMP和ADP的.
阿尔多酶充当FBP传感器,启动该途径.
抑制TRPV通道和改变 lysosomal pH 是关键的.
甲胺和曼诺斯利用这种溶酶体通路.

结论:

存在一种独特的溶酶体AMPK激活途径,与正规机制不同.
这一途径由糖溶性中间体和离子通道活性调节.
了解这种途径可以了解代谢药物的作用.

关键词:

这就是AMPKK.在AXIN AXIN中使用.阿尔多酶的使用方法一个LKB1一个LKB1 lysosome 是一种溶解体.在Ragulator中使用Ragulator.TRPVV 在线播放这是一个v-ATPasease.

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