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克桑托莫纳斯·坎佩斯特里斯公司 (Xanthomonas campestris pv. 坎佩斯特里斯通过感知蓝光来调节毒性机制

V P Conforte^1,2, J Rinaldi^3,4, H R Bonomi^3,5

¹Instituto de Ciencia y Tecnología Dr. César Milstein (CONICET - Fundación Pablo Cassará), Saladillo 2468, C1440FFX, Buenos Aires, Argentina.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

|February 24, 2025

概括

克桑托莫纳斯·坎佩斯特里斯公司 (Xanthomonas campestris pv. 坎佩斯特里斯 (Xcc) 使用两个光受体来感知蓝光,影响细菌毒性因素,如运动性和生物膜形成. 这揭示了植物病原体中光介调节的新机制.

科学领域:

微生物学微生物学
植物病理学植物病理学
摄影生物学摄影生物学

背景情况:

光是影响细菌行为的关键环境因素.
克桑托莫纳斯·坎佩斯特里斯公司 (Xanthomonas campestris pv. 坎佩斯特里斯 (Xcc) 是一种致病原体,会导致十字花的黑色腐烂,它具有一种细菌染色体 (XccBphP),可以调节红色/远红光的毒性.

研究的目的:

研究蓝光光受体 (LOV-histidine kinase) 在Xcc.中的作用.
为了确定XccBphP是否也能感知到蓝光.
为了阐明蓝光对Xcc毒性因子的影响.

主要方法:

基因操纵 (敲除和补充菌株) 来评估XccLOV的功能.
在不同的光照条件下分析游泳运动性和桑坦生产.
微镜检查生物膜结构和毒性测定.

主要成果:

无论是XccBphP还是XccLOV都能感知到蓝光.
蓝光对Xcc游泳运动有负面影响.
克桑的产生是由XccBphP独立于蓝光调节的.
蓝光改变了生物膜结构,增强了Xcc的毒性.

结论:

Xcc利用至少两个光受体,XccBphP和XccLOV,在对蓝光的反应中调节毒性.
蓝光影响细菌的运动性,生物膜的形成和Xcc.的致病性.

关键词:

克桑托莫纳斯·坎佩斯特里斯公司 (Xanthomonas campestris pv. 坎佩斯特里斯的营地在这种情况下,细菌菌菌素.生物膜是一种生物膜.蓝光是蓝色的光,蓝光是蓝光的.在EPS中使用EPS.固体葡萄糖酶是一种内分葡萄糖酶.感染感染感染低水平的胺基酶.游泳游泳是一种游泳方式.

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