内体化学基因受体信号体调节细胞迁移背后的中心机制
Hyunggu Hahn1,2, Carole Daly3, John Little1,4
1Department of Molecular Pathobiology, New York University College of Dentistry, New York, United States.
eLife
|February 24, 2025
概括
像CCR7这样的基因基因受体从内基因组中发出信号,招募G蛋白和β-arrestin. 这种内体体信号增强了Rho GTPase Rac1的活性,促进了免疫T细胞的迁移.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 化基因受体是一种G蛋白结合受体 (GPCR),对免疫和癌细胞迁移至关重要.
- 虽然已知血信号传递,但它们在内体体信号传递中的作用仍然不太了解.
- 其他受体类型的内体体信号影响细胞迁移,促使对化学因子受体进行研究.
研究的目的:
- 调查内体内基因因子受体信号传递在调节细胞迁移中的作用.
- 为了确定化基因受体是否可以从内体内激活信号通路.
- 为了确定特定的分子机制,将内体内基因因子受体活性与细胞运动联系起来.
主要方法:
- 利用药理学和细胞生物学方法研究化学因受体功能.
- 采用时空解析的APEX2蛋白质组分析来分析内分泌体中的蛋白质丰富.
- 测量了G蛋白和β-arrestin招募以及Rho GTPase活性.
主要成果:
- 证明化学受体CCR7在刺激时同时招募G蛋白和β-arrestin.
- 显示内化CCR7可以激活内体的G蛋白信号传递.
- 与血膜CCR7.7相比,在内体细胞CCR7中鉴定了Rho GTPase调节者的独特丰富.
- 链接内体CCR7信号传递以增强Rho GTPase Rac1活性和免疫T细胞化学反应.
结论:
- 内体化学基因受体信号传递在调节细胞迁移方面发挥着综合作用.
- 内部化化基因受体可以从内分泌体积极发出信号来调节细胞功能.
- 通过CCR7介导的内体信号增强了Rac1的活动,通过膜突起形成驱动T细胞化学反应.
相关概念视频
Chemotaxis and Direction of Cell Migration
3.3K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.3K
Cell Migration
16.9K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
16.9K
Cytoskeletal Coordination in Cell Migration
4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Role of Myosin in Cell Migration
2.2K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
2.2K
Cell Polarization by Rho Proteins
2.6K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.6K


