在棒光受体发育过程中,CTCF调节了全球染色质的可访问性和转录
Dahong Chen1, Saumya Keremane1, Silu Wang2
1Nuclear Organization and Gene Expression Section, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892.
概括
建筑蛋白CTCF在小鼠棒发育过程中调节全球基因表达和染色质可访问性. CTCF的枯竭显著改变了转录组和基因组的可访问性,突出了它的关键作用.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 发展生物学 发展生物学
背景情况:
- 染色体结构影响基因转录,但其在全球调节中的作用尚未完全理解.
- 以前的研究表明,在结构性蛋白质耗尽后,基因表达变化有限,可能是由于细胞异质性.
- 建筑蛋白CTCF在全球转录调节中的作用需要进一步研究.
研究的目的:
- 研究CTCF在全球染色质可访问性和基因表达调节中的作用.
- 为了解决在以前的研究中观察到的染色体组织变化和基因表达改变之间的差异.
- 为了阐明CTCF在同步开发过程中的功能,使用排序的鼠标棒.
主要方法:
- 进行了多omics分析,对被排序的青少年转移后小鼠棒进行了分析.
- 为了评估其对染色质可访问性和基因表达的影响,CTCF被耗尽.
- 分析的重点是欧克罗马丁的CTCF占用率及其与基因调节的相关性.
主要成果:
- CTCF耗尽导致了大约20%的转录组和41%的基因组可访问性的失调.
- 这些变化发生在观察到显著细胞表型之前.
- 结合euchromatin的CTCF表明了直接调节,CTCF促进了可访问性,但通常会抑制目标基因的表达.
结论:
- 在杆子发育过程中,CTCF在调节全球染色质可访问性和转录方面发挥着至关重要的作用.
- CTCF直接与活性位点结合,影响染色质的可访问性和基因表达,经常充当抑制剂.
- 细胞异质性和细胞类型特异性是多组体分析的关键考虑因素.
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