介酶干细胞通过降低十-十一转位1的调节,通过它们的分泌体调节乳腺癌的进展
Romina Motamed1, Keyvan Jabbari1, Mahboubeh Sheikhbahaei1
1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, P.O. Box: 14115-154, Tehran, Iran.
Scientific reports
|February 24, 2025
概括
介酶干细胞秘密体通过降低十-十一转位1 (TET1) 的调节来抑制乳腺癌 (BC) 的进展. 这种无细胞的方法对BC疗法有前途,并增强了思丁的敏感性.
科学领域:
- 在瘤学瘤学.
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
背景情况:
- 介酶干细胞 (MSCs) 通过其膜分泌体影响瘤微环境 (TME),对瘤生长产生上下文依赖的影响.
- 十一转位1 (TET1),一种DNA脱甲基酶,表现出致癌性质,特别是在三阴性乳腺癌 (TNBC) 中.
研究的目的:
- 为了研究MSC衍生的秘密对乳腺癌 (BC) 细胞行为的影响.
- 阐明TET1在MSC介导的BC进展调节中的作用.
主要方法:
- 人类骨髓衍生MSCs的分离和表征.
- 治疗BC细胞系 (MDA-MB-231,MCF-7) 的使用MSC秘密体.
- 西部斑点,RT-qPCR和siRNA介导的TET1敲击.
- 细胞增殖,迁移,细胞循环,细胞亡和化学敏感性的分析.
主要成果:
- 显著抑制了BC细胞的增殖和迁移,降低了生命力.
- 循环D1,c-Myc和N-cadherin的下调,随着E-cadherin表达的增加,表明EMT抑制.
- 通过MSC的分泌治疗,降低了BC细胞中TET1的表达.
- TET1敲击模仿了MSC分泌体效应,诱导细胞循环停止和细胞亡,并增加了西斯的敏感性.
结论:
- 通过降低TET1.1的调节,MSC衍生的秘密组有效地抑制BC细胞的进展.
- 在BC细胞的增殖,迁移和化学抵抗中,TET1起着至关重要的作用.
- MSC 机密体代表了BC 潜在的无细胞治疗策略,增强了化学敏感性.
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