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人类牛奶微生物群的个性化重建:在现实世界的环境中可行的方法

Leonardo Gonçalves Gomes¹, Lucas de Figueiredo Soveral¹, Izadora Borgmann Frizzo²

¹Laboratory of Immunoregulation, iREG, Department of Microbiology, Immunology, and Parasitology, Center for Dysbiosis Control, Federal University of Santa Catarina.

Journal of visualized experiments : JoVE

|February 25, 2025

概括

这项研究开发了一种方法,可以使用母乳 (MOM) 恢复被巴氏化供体母乳 (PDM) 中的有益细菌. 这个过程增强了非母乳养婴儿的PDM,支持他们的健康.

科学领域:

新生儿营养和微生物学
人类牛奶储存和加工

背景情况:

母乳 (MOM) 是新生儿的最佳营养.
纯化供体人乳 (PDM) 是一个重要的替代品,但在加工过程中会失去有益的微生物.
商业婴儿配方奶粉缺乏母乳的免疫和营养益处.

研究的目的:

开发和验证一个使用MOM恢复PDM微生物群的协议.
调整本协议以在新生儿护理机构临床实施.
为无法接受MOM的早产婴儿提供增强的PDM.

主要方法:

一个涉及PDM用10%MOM接种疫苗的协议.
在37°C下4小时内化注射牛奶 (IM).
微生物学分析以比较复制牛奶 (RM) 的微生物群与MOM.

主要成果:

在IM后化过程中,成功实现了细菌生长.
RM的微生物群概况与MOM的微生物群概况非常相似.
该协议在临床试验环境中证明了可行性.

结论:

开发的协议有效地恢复了PDM的微生物群.
这种方法提供了一种可行的方法来增强非母乳养新生儿的PDM.
恢复的PDM有可能改善脆弱婴儿的营养和免疫支持.

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