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Updated: May 5, 2026

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盘扩散和LiofilchemTM MTS带法与微稀释的对盘扩散和LiofilchemTM MTS带方法的比较评估,用于测试cefepime/enmetazobactam敏感性

Cécile Emeraud^1,2,3, Hippolyte De Swardt^1,2, Sandrine Bernabeu^1,2

¹Université Paris-Saclay, Inserm, CEA, Center for Immunology of Viral, Auto-immune, Hematological and Bacterial diseases » (IMVA-HB/IDMIT/UMRS1184), Team Resist, Fontenay-aux-Roses & Le Kremlin-Bicêtre, France.

The Journal of antimicrobial chemotherapy

|February 25, 2025

概括

磁盘扩散和渐变条带提供了可靠的测试cefepime/enmetazobactam,一个关键的抗生素对抗耐药的阴性细菌. 这些方法有助于临床决策治疗具有挑战性的感染.

科学领域:

临床微生物学临床微生物学
抗生素敏感性测试抗生素敏感性测试
传染性疾病传染性疾病

背景情况:

塞费皮姆 (Cefepime/enmetazobactam) 是一种新型组合抗生素,有效对抗多药耐药格兰氏阴性肠道细菌.
准确的敏感性测试对于指导cefepime/enmetazobactam的临床应用至关重要,特别是对于ESBL和OXA-48产生菌株.

研究的目的:

评估两种商业方法:光盘扩散和LiofilchemTM MTS梯度条.
为了比较它们与汁微稀释 (BMD) 的性能,Cefepime/enmetazobactam敏感性测试的既定黄金标准.

主要方法:

测试了291种耐卡巴胺的肠细菌分离物,其中包括194种卡巴胺酶生产物.
使用BMD,光盘扩散和LiofilchemTM MTS条进行了敏感性测试.
使用EUCAST和FDA 2025断点解释结果,根据ISO准则计算协议和错误率.

主要成果:

磁盘扩散显示了高度的分类一致 (93.8% EUCAST, 95.9% FDA),FDA断点 (1.9%) 的可接受的非常重大错误率.
利奥菲尔克TM MTS 条带实现了 88.3% 的基本一致性,偏差为 -8.9%.
这两种方法都准确地确定了OXA-48生产者的敏感性,但在最小抑制度断点附近存在局限性.

结论:

磁盘扩散和LiofilchemTM MTS梯度条纹是BMD的可靠替代方案,用于例行cefepime/enmetazobactam敏感性测试.
这些方法支持临床决策对由耐药格拉姆阴性细菌引起的感染.

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