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Radmila Milenkovska¹, Nikola Geskovski¹, Dushko Shalabalija¹

¹Institute of Pharmaceutical Technology, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Blv. Mother Theresa No. 45, 1000 Skopje, Republic of North Macedonia.

Beilstein journal of nanotechnology

|February 25, 2025

概括

这项研究开发了充满temozolomide (TMZ) 的功能化碳纳米载体,用于质母细胞瘤治疗. 这些纳米载体表现出增强的细胞毒性和放射敏感化作用,表明组合治疗的潜力.

科学领域:

纳米技术和材料科学材料科学
瘤学和癌症治疗疗法
生物医学工程生物医学工程

背景情况:

多形质母细胞瘤 (GBM) 仍然是一个具有挑战性的脑瘤,治疗选择有限.
泰莫佐洛米德 (TMZ) 是GBM的关键化疗剂,但其疗效可能受到药物输送和耐药性的限制.
碳纳米结构由于其独特的物理化学特性,具有作为药物输送载体的潜力.

研究的目的:

开发使用多壁碳纳米管 (MWCNTs) 和MWCNTs-石墨烯 (MWCNTs-G) 混合化合物的双功能碳纳米载体 (CNs).
将TMZ纳入这些CN中,用聚乙烯糖醇 (PEG) 和叶酸 (FA) 功能化,以增强药物循环,BBB交叉和向释放.
评估这些纳米载体配方在GBM细胞上的体外细胞毒性和放射敏感性.

主要方法:

用PEG6000和FA对MWCNTs和MWCNTs-G进行共价功能化.
将TMZ加载到功能化纳米载体上,然后进行物理化学表征 (例如尺寸,表面电荷,药物加载效率).
对U87MG GBM细胞的细胞毒性和放射敏感性作用的体外评估,包括对玛辐射后的评估.

主要成果:

成功合成具有高TMZ负载效率和适合穿越血脑瘤屏障 (BBTB) 的双功能CN.
从纳米载体配方中证明了双相和持续的TMZ释放配置文件.
无论是空白的还是装有TMZ的CN的显著度依赖性细胞毒性,混合MWCNTs-G配方的疗效更高. 当GBM细胞被CNs处理并随后照射时,观察到增强的细胞死亡,表明具有放射敏感性.

结论:

功能化的MWCNTs和MWCNTs-G纳米载体有效地装载和输送TMZ,在体外对GBM细胞表现出有希望的细胞毒性.
纳米载体配方表现出显著的放射敏感化作用,增强了玛辐射的疗效.
使用这些碳纳米载体与TMZ和放射治疗的联合治疗有可能改善GBM治疗的临床结果.

关键词:

碳纳米结构的碳纳米结构.细胞毒性细胞毒性质母细胞瘤多形具有辐射敏感性质的特性.泰莫索洛米德 (temozolomide) 是一种有毒的物质.

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