基于聚合诱导发光纳米探针的双模式免疫染色学试验条,用于在玉米中检测ZEN
Haitao Xu1,2,3, Chunlei Yu1,2,3, Keyun Ren1,2,3
1School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong 255049, China. yqqing@sdut.edu.cn.
Analytical methods : advancing methods and applications
|February 25, 2025
概括
使用聚合诱导排放聚合物微球的新型双模式试验带能够快速检测zearalenone (ZEN). 这种方法克服了传统光检测的局限性,为zearalenone分析提供了高灵敏度和可靠性.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 泽拉 (ZEN) 是一种广泛存在的真菌毒素,具有生殖性毒性,肝毒性和致癌性.
- 目前的快速ZEN检测方法受到光材料中聚合引起的火效应 (ACQ) 的阻碍.
- 对于新,灵敏和可靠的ZEN检测技术有着至关重要的需求.
研究的目的:
- 开发一种新的双模式 (色度计/光) 免疫染色学试验条,用于敏感的泽拉伦检测.
- 为了增强检测能力,合成和描述聚合诱导排放聚合物微球 (AIEPMs).
- 克服现有的光检测方法中ACQ效应的局限性.
主要方法:
- 合成包含聚合诱导排放 (AIE) 染料的AIE聚合物微球 (AIEPMs).
- 通过将AIEPM与针对ZEN的抗体结合起来,制备免疫试验.
- 使用基于AIEPM的免疫探针进行双模式检测的免疫染色体测试条 (ICA) 的开发.
- 测试参数的优化和使用标准解决方案和样本分析进行验证.
主要成果:
- 基于AIEPM的免疫染色学试验条实现了0.625 ng mL-1的视觉检测极限 (vLOD) 在色度模式下.
- 光模式的检测量极限 (qLOD) 显着较低,为0.039 ng mL-1.
- 该方法表现出极好的准确性,平均恢复率从97.60%到102.46%不等,可重复性高,变化系数 (CV) 在2.10%到10.70%之间.
结论:
- 开发的基于AIEPM的免疫染色学测试条提供了一种敏感和可靠的方法来检测泽拉.
- 双模式功能提供了灵活性和增强的检测极限,克服了ACQ的限制.
- 这种AIEPM-ICA方法显示出快速,准确和现场选zearalenone污染的巨大潜力.
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