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Updated: Jun 30, 2026

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
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电穿孔诱导基因表达的意想不到的变化:选择最佳传染方法的提示

Taiji Hamada1, Seiya Yokoyama1, Toshiaki Akahane1,2

  • 1Department of Pathology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8544, Japan.

Current issues in molecular biology
|February 25, 2025
PubMed
概括

电穿孔会影响U-251和U-87MG细胞中的基因表达,影响血小板衍生生长因子受体α (PDGFRA) 和受体氨酸激酶 (RTK). 再组合腺相关病毒 (rAAV) 显示的不良影响较少.

关键词:
这是PDGFRA.电穿孔的电穿孔是什么意思基因组编辑 基因组编辑脂肪过量 脂肪过量受体氨酸激酶基因受体的基因再组合腺相关病毒.

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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection10:34

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Transduction01:16

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Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...